棉花小GTP结合蛋白GhROP4与蛋白香叶酰基转移酶GhGGB的相互作用  

Interaction Between Cotton Small GTP Binding Protein GhROP4 and Protein Geranyl Acyltransferase GhGGB

作　　者：李月[1] 吾尕力汗·阿不都维力 代培红[1] 刘超[1] 姚正培[1] 郭旺珍[2] 刘晓东[1] Li Yue;Wugalihan Abuduwili;Dai Peihong;Liu Chao;Yao Zhengpei;Guo Wangzhen;Liu Xiao-dong(College of Agriculture,Xinjiang Agricultural University,Urumqi,830052;College of Agriculture,Nanjing Agricultural University,Nanjing,210095)

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]南京农业大学农学院,南京210095

出　　处：《分子植物育种》2020年第24期8126-8130,共5页Molecular Plant Breeding

基　　金：南京农业大学-新疆农业大学联合基金(KYYJ201701);国家自然基金项目(31660433)共同资助。

摘　　要：ROP蛋白广泛存在于高等植物细胞中,对调节植物生长发育、介导植物抵御逆境和激素信号转导发挥重要作用。GGB基因编码Ⅰ型蛋白,即香叶酰基转移酶,参与蛋白异戊二烯化修饰,在植物响应逆境胁迫过程中具有重要的调控作用。本实验室前期研究证明棉花GhROP4和GhGGB基因响应干旱、高盐、低温和棉花黄萎病病原菌的侵染应答。为了探究GhROP4和GhGGB蛋白之间的相互作用,本研究通过双分子荧光系统(bi-molecular fluorescence complementation, BiFC)实验,发现共转化表达GhROP4和GhGGB载体的本氏烟表皮细胞细胞膜上具有强烈的黄色荧光,表明这两个蛋白可以发生相互作用。研究结果为进一步明确GhROP4和GhGGB蛋白调控棉花抗逆机理提供理论依据。ROP proteins are widely present in higher plant cells. ROP proteins play an important role in regulating plant growth and development, coordinating plant resistance to stress and maintaining hormone signal transduction. The GGB gene encodes a type I protein geranyl acyltransferase, which is involved in the protein prenylation modification and takes part in plant response to stress. Our previous studies demonstrated that cotton GhROP4 and GhGGB genes are related to drought, high salt, low temperature and cotton verticillium infection. To investigate the relationship between GhROP4 and GhGGB proteins, the vector for BiFC was constructed. The strong yellow fluorescence signal of the interaction between GhROP4 and GhGGB was found to be distributed on the cell membrane of Nicotiana tabacum L. cells in the Bi-fluorescense complementation experiment, indicating the two proteins are interacted. The results provide an important theoretical basis for further study of GhROP4 and GhGGB regulating cotton stress resistance mechanism.

关 键 词：ROP蛋白 GGB基因 双分子荧光互补 棉花 抗逆 

分 类 号：S562[农业科学—作物学]