陆地棉亚鸡脚叶种质耐干旱胁迫分析  

Analysis of Tolerance to Drought Stress of Germplasm with Sub-okra Leaf Shape in Upland Cotton

作　　者：郑锦秀 姜辉[2] 王永翠[2] 柴启超 陈莹[2] 王秀丽[2] 高明伟[2] 王家宝[2] 张超[2] 赵军胜 Zheng Jinxiu;Jiang Hui;Wang Yongcui;Chai Qichao;Chen Ying;Wang Xiuli;Gao Mingwei;Wang Jiabao;Zhang Chao;Zhao Junsheng(School of Life Science,Shandong Normal University,Jinan,250014;Shandong Cotton Research Center,Jinan,250100)

机构地区：[1]山东师范大学生命科学学院,济南250014 [2]山东棉花研究中心,济南250100

出　　处：《分子植物育种》2020年第24期8273-8279,共7页Molecular Plant Breeding

基　　金：山东省自然科学基金(ZR2017BC077);山东省农业科学院青年科研基金(2016YQN09);山东省农业科学院农业科技创新工程(CXGC2018B01);国家现代农业产业技术体系(CARS-15-05)共同资助。

摘　　要：为系统研究亚鸡脚叶种质的耐干旱能力,评价其在棉花遗传改良中的应用价值,本研究用聚乙二醇6000(PEG6000)模拟干旱胁迫,测定了干旱胁迫下亚鸡脚叶和常态叶材料幼苗形态、光合参数、叶绿素含量及叶绿素荧光最大光化学效率(Fv/Fm)、超氧化物歧化酶(SOD)、过氧化物酶(POD)过氧化氢酶(CAT)及抗坏血酸(APX)等抗氧化酶的活性及可溶性糖、脯氨酸、丙二醛(MDA)含量的差异。结果表明在15%PEG胁迫下,亚鸡脚叶和常态叶材料的差异最明显,亚鸡脚叶材料的蒸腾速率(Tr)、气孔导度(Gs)、净光合速率(Pn)、细胞间隙二氧化碳浓度(Ci)、叶绿素含量及Fv/Fm在胁迫及复水后均优于常态叶材料。亚鸡脚叶材料的SOD、POD、CAT及APX抗氧化酶活性及调节渗透物质(可溶性糖和脯氨酸)在胁迫过程中均高于常态叶材料。这说明无论是光合生理特性,还是干旱胁迫生理响应,亚鸡脚叶材料都优于常态叶材料,表现出更好的耐旱性,这为棉花耐旱新品种选育和遗传改良提供了材料支撑。In o rder to e valuate the application of sub-okra leaf shape in cotton genetic improvement, this experiment was designed to study the drought tolerance of germplasm with sub-okra leaf shape. In this study, photosynthesis, chlorophyll content, chlorophyll fluorescence characteristic, activity of antioxidant enzymes, content of soluble sugar, dissociate proline and malondial dehyde(MDA) were determined in an experiment during continuous drought stress simulated with PEG 6000 and rehydration. The difference between materials with sub-okra leaf and normal leaf was the most obvious with the treatment of 15% PEG and subsequent rehydration. The transpiration rate(Tr), stomatal conductance(Gs), net photosynthetic rate(Pn), concentration of intercellular carbon dioxide(Ci), chlorophyll content and Fv/Fm of sub-okra leaf were better than those of normal leaf. The activities of antioxidant enzymes(SOD, POD, CAT and APX) and content of osmotic regulations(Soluble sugar and proline) in sub-okra leaf were higher than those in normal leaf. With better performance of photosynthetic physiological characteristics and physiological response, drought tolerance of germplasm with sub-okra leaf was superior to that of germplasm with normal leaf.

关 键 词：棉花 亚鸡脚叶 干旱 光合 

分 类 号：S562[农业科学—作物学]