白介素-38在活动期系统性红斑狼疮患者中的表达及与IKKβ激酶的关系  

The expression of IL-38 in the peripheral blood and its correlation with IKKβ kinase in active systemic lupus erythematosus patients

作　　者：张继云 赵旌[1,2] 努尔夏提·塔布什 罗莉[1,2] ZHANG Jiyun;ZHAO Jing;Nuerxiati·Tabushi;LUO Li(Department of Rheumatology and Immunology, the First Affiliated Hospital of Xinjiang Medical University, Wulumuqi 830054, China, Department of Rheumatology and Immunology, the Second Affiliated Hospital of Xinjiang Medical University, Wulumuqi 830063, China)

机构地区：[1]新疆医科大学第一附属医院风湿免疫科,乌鲁木齐830054 [2]新疆医科大学第二附属医院风湿免疫科,乌鲁木齐830063

出　　处：《暨南大学学报（自然科学与医学版）》2021年第1期48-53,共6页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：新疆维吾尔自治区自然科学基金项目(2019D01C239)。

摘　　要：目的:研究活动期系统性红斑狼疮(SLE)患者外周血中白介素-38(IL-38)和IKKβ激酶的表达水平,以探讨IL-38与IKKβ的关系.方法:采用实时荧光定量聚合酶链反应检测IL-38、IKK基因表达水平;酶联免疫吸附试验(ELISA)检测血清IL-38、肿瘤坏死因子α(TNF-α)蛋白表达水平;采用t检验或Mann-Whitney秩和检验.结果:①活动期SLE患者IL-38 mRNA表达水平(0.36±0.09)显著低于正常对照组(1.00±0.17),差异有统计学意义(P<0.01);活动期SLE患者IL-38蛋白表达水平(5.86±2.76)pg/mL显著低于正常对照组(18.48±1.35)pg/mL,差异有统计学意义(P<0.05);②活动期SLE患者TNF-α表达水平(10.78±1.25)pg/mL显著高于正常对照组(4.34±0.69)pg/mL,差异有统计学意义(P<0.05);③活动期SLE患者IKKβmRNA表达水平(6.01±1.51)显著高于正常对照组(1.16±0.14),差异有统计学意义(P<0.05);④活动期SLE患者IL-38与TNF-α蛋白表达水平呈负相关,差异有统计学意义(P<0.05);⑤活动期SLE患者IL-38与IKKβmRNA表达水平呈负相关,差异有统计学意义(P<0.05).结论:活动期SLE患者IL-38基因及蛋白表达水平下降,并且与TNF-α和IKKβ水平呈负相关,推测在SLE患者体内IL-38水平下降,促进TNF-α和IKKβ的高表达,从而活化核因子-κB(NF-κB)信号通路,参与了SLE的发病.Objective:To study the expression of interleukin-38(IL-38)and IKKβkinase in peripheral blood of patients with active systemic lupus erythematosus(SLE),and to explore the relationship between IL-38 and IKKβ.Methods:The gene expression levels of IL-38 and IκB kinase complex were detected by real-time fluorescence quantitative polymerase chain reaction(PCR),serum IL-38 and tumor necrosis factor-α(TNF-α)levels were detected by enzyme-linked immunosorbent assay(ELISA),and t-test or Mann-Whitney rank sum test were used.Results:The expression level of IL-38 mRNA in active SLE patients(0.36±0.09)was significantly lower than that in the normal control group(1.00±0.17),and the difference was statistically significant(P<0.01).The expression level of IL-38 protein in active SLE patients(5.86±2.76)was significantly reduced as compared with normal control group(18.48±1.35),and the difference was statistically significant(P<0.05).Expression of TNF-αin patients with active SLE(10.78±1.25)was significantly higher than that in the normal control group(4.34±0.69),the difference was statistically significant(P<0.05).The expression level of IKKβmRNA(6.01±1.51)in active SLE patients was significantly higher than that in normal control group(1.16±0.14),the difference was statistically significant(P<0.05).There was a negative correlation between the expression of IL-38 and TNF-α(P<0.05)and between the expression of IL-38 mRNA and IKKβmRNA(P<0.05)in patients with active SLE.Conclusion:The level of IL-38 gene and protein expression in active SLE patients was decreased,and negatively correlated with the level of TNF-αand IKKβ.It is speculated that the decrease of IL-38 level in SLE patients can promote the high expression of TNFαand IKK,thus causing the activation of NF-κB signaling pathway,which is involved in the pathogenesis of SLE.

关 键 词：系统性红斑狼疮 白介素-38 肿瘤坏死因子-Α IKKβ激酶 

分 类 号：R593.241[医药卫生—内科学]