基于抗风湿关节炎相关微量元素生物可利用性优化制川乌和白芍配伍研究  

Anti-arthritic-related metal bioavailability for optimal compatibility ratio of Aconiti Radix Cocta and Paeoniae Radix Alba

作　　者：林路秀[1,2,3] 郑凤英 黄桂珍[4] 李顺兴 刘凤娇[1,3] 满珊[3] 吴济如 李莹莹[3] LIN Lu-xiu;ZHENG Feng-ying;HUANG Gui-zhen;LI Shun-xing;LIU Feng-jiao;MAN Shan;WU Ji-ru;LI Ying-ying(Fujian Provincial Key Laboratory of Pollution Monitoring and Control,Minnan Normal University,Zhangzhou 363000,China;Fujian Provincial Key Laboratory of Modern Analytical Science and Separation Technology,Minnan Normal University,Zhangzhou 363000,China;College of Chemistry,Chemical Engineering and Environment,Minnan Normal University,Zhangzhou 363000,China;MinXi Vocational&Technical College,Longyan 364021,China)

机构地区：[1]闽南师范大学福建省现代分离分析科学与技术重点实验室,福建漳州363000 [2]闽南师范大学福建省污染监测与控制省高校重点实验室,福建漳州363000 [3]闽南师范大学化学化工与环境学院,福建漳州363000 [4]闽西职业技术学院,福建龙岩364021

出　　处：《中国中药杂志》2020年第23期5770-5776,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(21675077,22074058);福建省高校产学合作项目(2019Y4010);福建省教育厅中青年项目(JAT170341)。

摘　　要：微量元素缺乏或过量与类风湿关节炎(rheumatoid arthritis,RA)的病因和发病机制有关,均衡补充微量元素有助于RA治疗。制川乌和白芍配伍被认为是治疗RA较安全的药物。该文旨在测定制川乌和白芍单煎液及其配伍水煎液中RA相关元素[锰(Mn)、铁(Fe)、铜(Cu)、锌(Zn)和硒(Se)]的生物可利用性,据此优化配伍比例。应用体外全消化道(口腔、胃、小肠和大肠)全功能仿生模型,模拟单煎液及不同配伍水煎液在人体消化系统消化和代谢,探讨胃肠消化酶和肠道菌群的作用;应用单层脂质体为细胞生物膜模型,体外模拟胃肠吸收,以脂质体亲和的金属含量评价RA相关金属的生物可利用性。研究表明,RA相关微量元素生物可给性和生物可利用性与配伍比例、胃肠仿生消化和肠道微生物代谢三者均具有显著性差异(P<0.05),即影响RA相关微量元素在胃肠道中释放和吸收。基于RA相关金属的最高生物利用率,推荐制川乌(A)和白芍(B)的最佳配伍比例为1A∶1B,与临床常用配比吻合,证明RA相关金属生物可利用性可能是药效的基础。Trace metals deficiency or excess are associated with the etiology and pathogenesis of rheumatoid arthritis(RA).Aconiti Radix Cocta(A)and Paeoniae Radix Alba(B)are commonly used together for the treatment of RA.In this study,we aim to determine anti-arthritic-related metal bioavailability in the compatibility of herb A and B for avoiding metal deficiency or excess,and optimize the combination ratio of herb A and B,accordingly.Anti-arthritic-related metal bioaccessibility were evaluated by in vitro simulator of all gastrointestinal tract(including mouth,stomach,small and large intestines),and the roles of gastrointestinal digestive enzymes and intestinal microflora were investigated.Anti-arthritic-related metal bioavailability was assessed by the affinity adsorption with liposomes.The results indicated that compatibility proportion of corresponding herbal plants,gastrointestinal digestion and microbial metabolic,which could affect metal digestion and absorption.The optimal compatibility proportion of 1 A∶1 B is recommended,according to the dose of anti-arthritic-related metal bioavailability,which is often chosen for clinical practice of RA therapy.Thus,anti-arthritic-related metal bioavailability might be the key active substances for RA treatment.

关 键 词：中草药配伍 类风湿关节炎 微量元素 生物可利用性 体外仿生模型 制川乌 白芍 

分 类 号：R285[医药卫生—中药学]