抗幽门螺杆菌黏附素蛋黄抗体与HP1188蛋黄抗体在小鼠体内防治幽门螺杆菌感染的比较  

Comparative study of HpaA-IgY and HP1188-IgY for prevention and treatment of Helicobacter pylori infection in mice

作　　者：韩飞 张帅帅 HAN Fei;ZHANG Shuai-Shuai(Department of Medical Laboratory Three Gorges Hospital of Chongqing University,Chongqing 404100,China)

机构地区：[1]重庆大学附属三峡医院医学检验科,重庆404100

出　　处：《中国生物制品学杂志》2020年第12期1398-1402,1408,共6页Chinese Journal of Biologicals

基　　金：重庆市自然科学基金(cstc2019jcyj-msxm X0631);重庆市卫生局计划项目(2011.2.597);重庆市万州区科委重点科技计划项目(201203006)。

摘　　要：目的比较抗幽门螺杆菌黏附素(Helicobacter pylori adhesin A,HpaA)蛋黄抗体(immunoglobulin yolk,IgY)(HpaA-IgY)及抗H.pylori HP1188 IgY(HP1188-IgY)在BALB/c小鼠体内预防和治疗H.pylori感染的最低浓度,并评价治疗效果。方法Western blot鉴定HpaA-IgY及HP1188-IgY抗原特异性。制备1×10^9 CFU/mL H.pylori菌液,将BALB/c小鼠随机分为阳性对照组(灌喂H.pylori菌液0.5 mL)、阴性对照组(以布氏肉汤替换H.pylori)、预防组(先灌喂1、3、5 mg/mL HpaA-IgY或HP1188-IgY及PBS各1 mL,再灌喂H.pylori菌液0.5 mL)、治疗组(先灌喂H.pylori菌液0.5 mL,再灌喂1、3、5 mg/mL HpaA-IgY或HP1188-IgY及PBS各1 mL)。处理完成后,取各组小鼠胃组织进行H.pylori培养、快速尿素酶试验(rapid urease test,RUT)及组织学检查,观察H.pylori定植及炎症反应情况。结果经Western blot分析,HpaA-IgY及HP1188-IgY分别与重组HpaA和HP1188蛋白发生特异性结合。阳性及PBS对照组小鼠感染率均为100%。与PBS对照组比较,5 mg/mL HpaA-IgY及3 mg/mL HP1188-IgY预防组和治疗组感染率均降低,RUT、H.pylori定植及炎症程度评分均显著降低(P均<0.05),均达到较理想的预防和治疗效果。结论HpaA-IgY及HP1188-IgY对感染H.pylori小鼠均有预防和治疗双重作用,剂量选择HP1188-IgY优于HpaA-IgY。本文为制备防治H.pylori感染的口服生物制品提供了参考。Objective To compare the minimum concentrations of immunoglobulin yolk(IgY)against Helicobacter pylori adhesin A(HpaA)and H.pylori HP1188 in prevention and treatment of H.pylori infection in BALB/c mice and evaluate the curative effect.Methods Western blot was used to analyze the specificities of HpaA-IgY and HP1188-IgY antigens.H.pylori suspension at a concentration of 1×10^9 CFU/mL was prepared.BALB/c mice were randomly divided into various groups.The mice in positive control group were fed with 0.5 mL H.pylori suspension,while those in negative control group with Brinell broth,those in prevention groups with 1,3 and 5 mg/mL HpaA-IgY,HP1188-IgY and PBS respectively,1 mL for each,followed by 0.5 mL H.pylori suspension,and those in treatment groups with 0.5 mL H.pylolri suspension followed by 1,3 and 5 mg/mL HpaA-IgY,HP1188-IgY and PBS respectively,1 mL for each.The gastric tissues of mice in various groups were subjected to H.pylori culture,rapid urease test(RUT)and histological examination to observe the H.pylori colonization and inflammatory reaction.Results Western blot showed specific binding of HpaA-IgY and HP1188-IgY to recombinant HpaA and HP1188 proteins respectively.Both the infection rates of mice in positive and negative control groups were 100%.The infection rate as well as scores for RUT,H.pylori colonization and inflammatory reaction in the mice prevented and treated with 5 mg/mL Hpa-IgY and 3 mg/mL HP1188-IgY decreased significantly as compared with those with PBS(each P<0.05),indicating satisfactory preventive and curative effects.Conclusion Both HpaA-IgY and HP1188-IgY showed preventive and curative effects on H.pylori infection in mice,while the later was superior to the former in dose selection.

关 键 词：幽门螺杆菌 抗幽门螺杆菌黏附素 HP1188蛋白 蛋黄抗体 体内试验 

分 类 号：R378.99[医药卫生—病原生物学]