HPLC法测定软脉灵口服液中毛蕊花糖苷、焦地黄苯乙醇苷B1、迷迭香酸、紫草酸、丹酚酸B、2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷和虎杖苷  

Determination of verbascoside,jionoside B1,rosmarinic acid,lithospermic acid,salvianolic acid B,2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside,and polydatin in Ruanmailing Oral Liquid by HPLC

作　　者：马伟国 马灵珍 MA Wei-guo;MA Ling-zhen(Department of Pharmacy,Xinmi Hospital of TCM,Zhengzhou 452370,China;School of Pharmacy,Bozhou Vocational and Technical College,Bozhou 236800,China)

机构地区：[1]新密市中医院药剂科,河南郑州452370 [2]亳州职业技术学院药学院,安徽亳州236800

出　　处：《现代药物与临床》2020年第12期2321-2325,共5页Drugs & Clinic

基　　金：安徽省教育厅高校自然科学研究项目(KJ2017A770)。

摘　　要：目的建立HPLC法测定软脉灵口服液中毛蕊花糖苷、焦地黄苯乙醇苷B1、迷迭香酸、紫草酸、丹酚酸B、2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷和虎杖苷。方法采用Venusil XBP C18色谱柱(250 mm×4.6 mm,5µm);以乙腈–0.1%甲酸水溶液为流动相,梯度洗脱;检测波长分别为330 nm(0~17 min检测毛蕊花糖苷和焦地黄苯乙醇苷B1)、280 nm(17~50 min检测迷迭香酸、紫草酸、丹酚酸B、2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷和虎杖苷);体积流量1.0 mL/min;柱温30℃;进样量10µL。结果毛蕊花糖苷、焦地黄苯乙醇苷B1、迷迭香酸、紫草酸、丹酚酸B、2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷和虎杖苷分别在1.23~30.75、0.59~14.75、0.71~17.75、1.47~36.75、9.78~244.50、6.57~164.25、1.19~29.75μg/mL线性关系良好(r≥0.9991);平均回收率分别为99.14%、97.62%、96.84%、98.96%、100.11%、99.46%、98.92%,RSD值分别为1.15%、1.27%、1.02%、0.96%、0.65%、1.41%、0.87%。结论该方法操作简便、重复性好,为软脉灵口服液中多指标质量评价和临床应用提供参考依据。Objective To establish an HPLC method for determination of verbascoside,jionoside B1,rosmarinic acid,lithospermic acid,salvianolic acid B,2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside,and polydatin in Ruanmailing Oral Liquid.Methods Venusil XBP C18 column(250 mm×4.6 mm,5μm)was used with acetonitrile-0.1%formic acid as mobile phase with gradient elution.The detection wavelength were set at 330 nm(for verbascoside and jionoside B1)and 280 nm(for rosmarinic acid,lithospermic acid,salvianolic acid B,2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside,and polydatin).The column temperature was 30℃,flow rate was set at 1.0 mL/min,and volume was 10µL.Results Verbascoside,jionoside B1,rosmarinic acid,lithospermic acid,salvianolic acid B,2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside,and polydatin showed good linear relationships within the ranges of 1.23—30.75,0.59—14.75,0.71—17.75,1.47—36.75,9.78—244.50,6.57—164.25,and 1.19—29.75μg/mL(r≥0.9991).The average recoveries of those components were 99.14%,97.62%,96.84%,98.96%,100.11%,99.46%,and 98.92%with the RSD values of 1.15%,1.27%,1.02%,0.96%,0.65%,1.41%,and 0.87%,respectively.Conclusion The method is simple and repeatable,which provides reference for the quality evaluation and clinical application of Ruanmailing Oral Liquid.

关 键 词：软脉灵口服液 毛蕊花糖苷 焦地黄苯乙醇苷B1 迷迭香酸 紫草酸 丹酚酸B 2 3 5 4’-四羟基二苯乙烯-2-O-β-D葡萄糖苷 虎杖苷 高效液相色谱 

分 类 号：R286.02[医药卫生—中药学]