不同毛色绵羊皮肤组织中MC1R、Agouti及TYR基因差异表达研究  

Expression and Regulation Analysis of TYR,MC1R and Agouti Genes in Sheep Skins with Different Coat Color

作　　者：薛丽娜 罗惠娣[1] 周胜花[1] 王曦[1] 毛杨毅[1] XUE Lina;LUO Huidi;ZHOU Shenghua;WANG Xi;MAO Yangyi(College of Animal Science,Shanxi Agricultural University,Shanxi Taigu 030032,China)

机构地区：[1]山西农业大学动物科学学院,山西太谷030032

出　　处：《中国畜牧杂志》2021年第1期91-95,100,共6页Chinese Journal of Animal Science

基　　金：国家绒毛用羊产业技术体系建设专项基金(CARS-39-24);山西省科技创新团队基金(201705D131028-20);山西省农科院农业产业发展科技引领工程项目资金(2019CYYL-12);山西省科技攻关项目(20120311024-1);山西省重点研发计划项目(201903D221003)。

摘　　要：本研究旨在探究特定杂交模式下产生全黑被毛绵羊TYR、MC1R及Agouti基因互作调控毛色的机制。随机选取黑色和白色被毛绵羊各4只,采集皮肤组织,利用qRT-PCR技术测定MC1R、Agouti及TYR基因mRNA在不同毛色绵羊皮肤中的表达量。结果显示:MC1R、Agouti及TYR基因在不同毛色绵羊皮肤中均有表达,其中TYR基因mRNA在黑色绵羊皮肤中的表达量极显著高于白色绵羊皮肤(P<0.01),MC1R基因mRNA在黑色绵羊皮肤中的表达量高于白色绵羊皮肤,但差异不显著,Agouti基因mRNA在黑色绵羊皮肤中的表达量显著低于白色绵羊皮肤(P<0.05)。综上,该杂交模式下产生的黑色绵羊可能是由于Agouti基因表达量低而使α-MSH诱导信号通路处于激活状态,上调了TYR基因表达量,导致真黑素含量上升,出现黑色毛色性状。The aim of this study was to explore the mechanism by which TYR,MC1 R and Agouti genes in black sheep interact to regulate coat color under special hybridization mode.Four black and white sheep of the same age were randomly selected to collect skin tissue,and the mRNA expression of TYR,MC1 R and Agouti genes in sheep skin with different coat color were analyzed by qRT-PCR.The results showed that TYR,MC1 R and Agouti mRNA were expressed in all sheep skin samples tested,and the expression of MC1 R mRNA in the skin of black sheep was higher than that of white sheep,but the difference was not significant(P>0.05).The expression of TYR mRNA in black sheep was significantly higher than that in white sheep(P<0.01),and the expression of Agouti mRNA in black sheep was significantly lower than that in white sheep(P<0.05).In conclusion,the black sheep produced in this hybridization mode may be due to the low Agouti expression,which activates theα-MSH signaling pathway,up-regulates the TYR expression,and leads to the increase of eumelanin content and the appearance of black coat color.

关 键 词：绵羊 毛色 TYR MC1R AGOUTI QRT-PCR 

分 类 号：S826.2[农业科学—畜牧学]