不同浓度呋喃酮C-30对鲍曼不动杆菌ATCC19606菌毛及生物被膜作用的研究  

Effects of different concentrations of furanone C-30 on pilus and biofilm formation of Acinetobacter baumannii ATCC19606

作　　者：刘聪蕊 敬蓉[1] 张伟[1] 柯蕊[1] 冯雁京 和平[1] 马淑珍[1] 赵玉杰[1] 杨妮[1] 刘原[1] Liu Cong-rui;Jing Rong;Zhang Wei;Ke Rui;Feng Yan-jing;He Ping;Ma Shu-zhen;Zhao Yu-jie;Yang Ni;Liu Yuan(Department of Respiratory and Critical Care Medicine,the Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004)

机构地区：[1]西安交通大学第二附属医院,西安710004

出　　处：《中国抗生素杂志》2020年第11期1186-1191,共6页Chinese Journal of Antibiotics

基　　金：国家自然科学基金(No.81270061)。

摘　　要：目的通过分析鲍曼不动杆菌菌毛编码及调节基因的表达情况,探究不同浓度呋喃酮C-30对鲍曼不动杆菌ATCC19606菌毛及生物被膜形成的作用。方法聚合酶链反应II检测菌株菌毛编码及调节基因的表达情况。不同浓度呋喃酮C-30下Real-time法检测鲍曼不动杆菌ATCC19606菌毛编码和调节基因表达水平、透射电镜下菌毛结构以及结晶紫染色法检测生物被膜的量。结果 csuA/B、csuA、csuB、csuC、csuD、csuE、bfmS和bfmR基因扩增阳性率分别为100%、7.14%、78.57%、89.29%、39.29%、82.14%、85.71%和85.71%。1/4MIC和1/2MIC浓度的呋喃酮C-30能够显著抑制鲍曼不动杆菌ATCC19606菌毛编码及调节基因的表达和菌毛、生物被膜的形成,浓度越大、抑制作用越明显。结论呋喃酮C-30能够抑制鲍曼不动杆菌ATCC19606菌毛编码及调节基因的表达和菌毛、生物被膜的形成,且存在一定的浓度依赖效应,此作用为新型抗菌药物的研究提供了方向。Objective To test the carrying capacity of pilus coding and regulatory genes in Acinetobacter baumannii strains. To study the effects of different concentrations of furanone C-30 on pilus and biofilm formation of Acinetobacter baumannii ATCC19606 strain. Methods Polymerase chain reaction(PCR) was used to test the expression of pilus coding and regulatory genes. The Real-time PCR method and transmission electron microscopy and the crystal-violet staining method were applied to detect the expression levels of pilus coding and regulatory genes, the pilus structure, and the ability of biofilm of Acinetobacter baumannii ATCC19606 with different concentrations of furanone C-30. Results The positive rates of csuA/B, csuA, csuB, csuC, csuD, csuE, bfmS and bfmR genes were 100%, 7.14%, 78.57%, 89.29%, 39.29%, 82.14%, 85.71% and 85.71%, respectively. The 1/4 MIC and the 1/2 MIC of furanone C-30 could effectively inhibit the expression of pilus coding and regulatory genes and the synthesis of pilus and biofilm of Acinetobacter baumannii ATCC19606. The greater the concentration, the more obvious the inhibition. Conclusion Furanone C-30 could inhibit the expression of the pilus coding and regulatory genes of Acinetobacter baumannii ATCC19606, resulting in the reduction of pilus and biofilm formation. There was a concentration-dependent effect. The results of this experiment provided research direction for the new drug research.

关 键 词：呋喃酮C-30 鲍曼不动杆菌 生物被膜 菌毛 群体感应系统 

分 类 号：R978[医药卫生—药品]