针灸“肺俞”“大椎”“风门”对哮喘模型大鼠肺组织中嗜酸性粒细胞凋亡的影响  

Influence of Needling and Moxibustion on BL13,DU14 and BL12 to Eosinophils Apoptosis in Lung Tissue of Asthma Model Rats

作　　者：郑洁 邵素菊[1,2] 何竹青 ZHENG Jie;SHAO Suju;HE Zhuqing(Henan University of Chinese Medicine, Zhengzhou 450046, China;The Third Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450008, China)

机构地区：[1]河南中医药大学,河南郑州450046 [2]河南中医药大学第三附属医院,河南郑州450008

出　　处：《中医药信息》2021年第1期38-42,共5页Information on Traditional Chinese Medicine

基　　金：河南省科技厅重点科技攻关项目(182102310108);国家中医药管理局河南邵氏针灸流派传承工作室第二期建设项目(国中医药人教函〔2019〕62号);河南省中医药科学研究专项(2015ZY02056)。

摘　　要：目的:观察针灸“肺俞”“大椎”“风门”对哮喘模型大鼠肺组织中嗜酸性粒细胞凋亡的影响。方法:将40只SD大鼠随机分为空白组、模型组、针刺组、艾灸组,每组10只。用卵蛋白对大鼠致敏、激发,建立哮喘模型,各组分别给予相应干预后,取大鼠右肺中叶组织,固定、包埋、切片。HE染色法观察各组大鼠肺组织病理形态变化;标记嗜酸性粒细胞的组化染色法观察肺组织中嗜酸性粒细胞表达;末端脱氧核苷酸转移酶介导的原位末端标记技术(TUNEL法)检测肺组织中嗜酸性粒细胞凋亡。结果:与空白组相比,模型组大鼠支气管管腔明显缩窄,支气管及肺间质内有大量炎性细胞浸润;肺组织内EOS计数明显增高,EOS凋亡率显著降低(均P<0.01);与模型组相比,针刺组与艾灸组大鼠支气管管腔轻度缩窄,支气管及肺间质内炎性细胞浸润较少,肺组织内EOS计数明显降低,EOS凋亡率显著增高(均P<0.01);针刺组与艾灸组相比,肺组织病理变化基本一致,嗜酸性粒细胞总数与其凋亡率比较差异均无统计学意义(均P>0.05)。结论:针灸可以通过诱导EOS凋亡,减轻EOS在气道内浸润,从而控制哮喘炎症反应。Objective:To observe the influence of needling and moxibustion on Feishu(BL13),Dazhui(DU14)and Fengmen(BL12)to eosinophils(EOS)apoptosis in lung tissue of asthmatic rats.Methods:40 SD rats were randomly divided into the blank group,the model group,the acupuncture group and the moxibustion group,with 10 rats in each group.The asthma model was established by sensitization with OVA in rats.After each group was given relevant treatment,the middle lobe tissue of the right lung was taken,fixed,embedded and sliced.HE staining was used to observe the pathological changes,histochemical staining was used to observe the level of EOS,and TUNEL method was used to observe the apoptosis of EOS.Results:Compared to the blank group,the bronchial lumen was significantly narrowed,and there was a large number of inflammatory cell infiltration in the bronchial tube and pulmonary interstitium,the EOS count in the lung tissue was significantly increased,the apoptosis rate of EOS was significantly decreased in the model group(P<0.01).Compared to the model group,the bronchial lumen was slightly narrowed,the infiltration of the inflammatory cells in the bronchial tube and pulmonary interstitium became less,the EOS count in the lung tissue was obviously reduced,and the apoptosis rate of EOS was obviously increased in the acupuncture group and the moxibustion group(P<0.01).There were no statistical differences in the pathological changes of the lung tissue,EOS count and the apoptosis rate of EOS between the acupuncture group and the moxibustion group(P>0.05).Conclusion:Acupuncture and moxibustion can induce the apoptosis of EOS and reduce EOS infiltration in airway,so as to control inflammatory reaction of asthma.

关 键 词：哮喘 针灸 嗜酸性粒细胞凋亡 

分 类 号：R246.1[医药卫生—针灸推拿学] R259[医药卫生—中医临床基础]