粗鳞蜡螟(Trachylepidia fructicassiella)DNA条形码研究  

Study on the DNA Barcode of Trachylepidia fructicassiella Ragonot

作　　者：陈光辉[1,2] 杨燕 张京宣 李焱 胡红英 Chen Guanghui;Yang Yan;Zhang Jingxuan;Li Yan;Hu Hongying(College of life Science and Technology,Xinjiang University,Urumqi Xinjiang 830046.China;College of Life and Geographic Sciences,Kashi University,Kashi Xinjiang 844000,China;Bureau of agriculture and water resources of Laoshan,Qingdao Shandong 266000.China;Qingdao Customs Technology Center,Qingdao Shandong 266000,China)

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046 [2]喀什大学生命与地理科学学院,新疆喀什844000 [3]山东省青岛市崂山区农业和水利局,山东青岛266100 [4]青岛海关技术中心,山东青岛266000

出　　处：《中国植保导刊》2020年第12期15-22,共8页China Plant Protection

基　　金：国家自然科学基金(U1170305)(31360523);原国家质检总局课题(2016IK194)

摘　　要：对腊肠树果实中的重要潜在外来有害生物粗鳞蜡螟(Trachylepidia fructicassiella Ragonot)的幼虫和成虫进行了DNA条形码研究.通过提取粗鳞蜡螟幼虫和成虫样品的基因组DNA,设计ITS1、ITS2、16S rRNA、CYT-B、COⅠ、COⅡ基因引物,PCR扩增并测序,结合Genbank中相关数据,借助生物信息学分析软件对比分析目的基因序列相似性,构建NJ系统进化树,分析遗传进化关系,获取该虫DNA条形码。结果表明,从粗鳞蜡螟幼虫和成虫的快速准确鉴定,获得了其幼虫和成虫多个基因的DNA序列,共计16条。其中,14条为新序列,分析表明这些序列可作为粗鳞蜡螟新的DNA条形码序列,为该虫的识别鉴定和幼虫的早期防治提供基础数据。The DNA barcodes of the larvae and adults of Trachylepidia fructicassiella, an important potential alien pest for China, were studied to facilitate its rapid and accurate identification. We extracted the genomic DNA of larvae and adult samples and amplified the ITS1, 16 S rRNA, CYT-B, COⅠ, and COⅡ genes by PCR. The amplified products were sequenced and aligned to GenBank sequences. The unknown larvae and adult samples were identified as Trachylepidia fructicassiella through COI sequence alignment, and 14 out of the 16 sequences amplified were new to the database.Sequence divergences were calculated and a neighbor-joining tree of distances was created. The DNA barcodes of this species were obtained by evolutionary relationships analysis. The results showed that these new sequences of ITS1, ITS2,16 S rRNA, CYT-B, and COII could be used as DNA barcodes of Trachylepidia fructicassiella for identification and early control of the larvae.

关 键 词：粗鳞蜡螟 DNA条形码 快速鉴定 ITS1、ITS2、CYT-B、COⅠ基因 

分 类 号：Q969.92[生物学—昆虫学]