桔绿木霉Snef1910联合噻唑膦防治根结线虫减施增效研究  

Application of Trichoderma citrinovirid Snef1910 in Reducing Application and Increasing Efficiency of Fosthiazate against Tomato Root Knot Nematode Disease

作　　者：刘晓宇[1] 王媛媛[1] 范海燕 段玉玺[1] LIU Xiao-yu;WANG Yuan-yuan;FAN Hai-yan;DUAN Yu-xi(College of Plant Protection/Nematology Institute of Northern China,Shenyang Agricultural University,Shenyang 110161,China)

机构地区：[1]沈阳农业大学植物保护学院/北方线虫研究所,沈阳110161

出　　处：《沈阳农业大学学报》2020年第6期755-761,共7页Journal of Shenyang Agricultural University

基　　金：“十三五”国家重点研发计划项目(2016YFD0201004);辽宁省教育厅高等学校基本科研项目(LSNQN201722)。

摘　　要：根结线虫病是设施蔬菜的主要土传病害之一,随着种植面积的逐年增加,根结线虫病害也日趋严重。噻唑膦是常用化学杀线剂。前期筛选获得1株对南方根结线虫有高毒力的生防真菌桔绿木霉(Trichoderma. citrinovirid)Snef1910。温室田间试验通过改变两者的施用次数和施用顺序,测试桔绿木霉Snef1910联合噻唑膦共同防治根结线虫的效果,以期实现噻唑膦在实际生产中的减施增效。试验共设置6个处理:snef1910仅在定植期施用1次(snef1910-1);噻唑膦仅在定植期施用1次(F-1);snef1910在定植期和30d后各施用1次(snef1910-2);噻唑膦在定植期和30d后各施用1次(F-2);snef1910在定植期施用1次,30d之后施用噻唑膦1次(snef1910+F);噻唑膦在定植期施用1次,30d之后施用1次snef1910(F+snef1910)。温室田间试验结果表明:F-2和F+Snef1910处理的防效最高为68.00%,snef1910-1,F-1,snef1910-2和snef1910+F处理的防效分别为32.00%、56.00%、36.00%和56.00%。F+snef1910处理的增产率为76.50%,显著高于其他处理。snef1910-1、F-1、snef1910-2、F-2和snef1910+F处理的增产率分别为37.56%、50.46%、47.93%、59.68%和58.18%。可见,定植期施用噻唑膦,生长期施用木霉Snef1910的顺序施用模式可以减少噻唑膦的使用次数,同时提高防效和产量,这是噻唑膦减施增效的有效办法。研究结果可为木霉联合其他化学杀线剂实现减施增效提供参考。Disease of root-knot nematode(RKN)is one of destructive soil-borne diseases in Greenhouse Vegetable Cultivation.RKN become more and more serious with the increasing plant area in China and made the serious economy loss.Fosthiazate is a chemical nematicide commonly used in controlling RKN disease.Snef1910 was screened with high efficacy against Meloidogyne incognita and identified as T.citrinovirid by our lab.By changing applied times and applied sequence,the effects of Snef1910 combined with fosthiazate on the control of root-node nematodes were studied,so as to realize the reducing application and increasing efficiency of fosthiazate against tomato root knot nematode disease in actual production.The experiment was set up at6 treatments:snef1910-1(Snef1910 was applied only once in establishment stage),F-1(fosthiazate was applied only once in establishment stage),snef1910-2(Snef1910 was applied in establishment stage and 30 days later),F-2(fosthiazate was applied in establishment stage and 30 days later),snef1910+F(Snef1910 was applied in establishment stage and fosthiazate was applied after30 days)and F+snef1910(fosthiazate was applied in establishment stage and Snef1910 was applied after 30 days).The results of plot experiment in greenhouse showed that the relative control effect of F+snef1910 could reach 68.00%,as well as F-2,which was higher than that of snef1910-1(32.00%),F-1(56.00%),snef1910-2(36.00%)and snef1910+F(56.00%).However,F+snef1910 increased the yield most significantly by 76.50%higher than snef1910-1,F-1,snef1910-2,F-2 and snef1910+F,which were37.56%,50.46%,47.93%,59.68%and 58.18%,respectively.These results suggested that it was an ordinal application pattern that fosthiazate was applied in establishment stage and Snef1910 was applied after 30 days which reduced the times of chemical agent fosthiazate and was an efficient approach to reduce the amount of fosthiazate and increase tomato yield in controlling RKN and could be used as a reference for reducing application and increasing efficiency of o

关 键 词：根结线虫病 桔绿木霉Snef1910 噻唑膦 农药减施增效 

分 类 号：S432.79[农业科学—植物病理学]