肠外营养混合液中维生素C含量测定的研究  

Study on the determination of vitamin C in total parenteral nutrition admixture

作　　者：穆殿平[1] 解晓帅[1] 张凤莹 许煜静[2] 杨金荣[2] Mu Dianping;Xie Xiaoshuai;Zhang Fengying;Xu Yujing;Yang Jinrong(Department of Pharmacy,Tianjin First Center Hospital,Tianjin 300192;School of Pharmacy,Tianjin Medical University,Tianjin 300070)

机构地区：[1]天津市第一中心医院,天津300192 [2]天津医科大学药学院,天津300070

出　　处：《天津药学》2020年第4期6-9,共4页Tianjin Pharmacy

基　　金：天津市第一中心医院基金项目(No.CF201822)。

摘　　要：目的:建立HPLC法测定肠外营养液(TNA)中维生素C(Vc)注射液含量的方法。方法:采用Zirchrom Kromasil C18(250 mm×4.6 mm,5μm)色谱柱;流动相:0.34%(w/v)磷酸二氢钾-氢氧化四丁基铵-乙腈(92.5∶2.5∶5,v/v/v,pH 3.0);流速:0.8 ml/min;检测波长:210 nm;柱温:30℃。结果:线性回归方程:Y=27105 X-10325(r=0.9998),线性范围为:50~600μg/ml;平均回收率分别为99.23%,RSD为1.06%;重复性RSD为1.78%。结论:该方法操作简单,快速准确,且分离度和重复性均较好,可用于成分复杂的TNA中Vc注射液的含量测定,同时也为进一步深入研究TNA中加入适宜浓度Vc注射液的可行性奠定了基础。Objective:To establish a high-performance liquid chromatography(HPLC)method for the determination of vitamin C(Vc)injection in total parenteral nutrition admixture(TNA).Methods:Zirchrom Kromasil C18(250 mm×4.6 mm,5μm)chromatographic column;Flow phase:0.34%(w/v)potassium dihydrogen phosphate/hydroxide tetrabutylammonium/acetonitrile(92.5,2.5,5,v/v/v,pH 3.0);Flow rate:0.8 ml/min;Detection wavelength:210 nm;Column temperature:30°C.Results:Linear regression equation:Y=27105X-10325(r=0.9998),linear range:50~600μg/ml;The average recovery rate was 99.23%(RSD 1.06%);Repetition RSD was 1.78%.Conclusion:The method is simple,fast,and accurate and has good separation and repeatability.It can be used for the determination of Vc injection in complex TNA.At the same time,it also laid a foundation for further study on the feasibility of adding an appropriate concentration of Vc injection into TNA.

关 键 词：肠外营养混合液(TNA) 维生素C(Vc)注射液 含量测定 

分 类 号：R927.2[医药卫生—药学]