光叶紫花苕(Vicia villosa Roth var.glabrescens)核型分析  

Karyotypes Analysis of Vicia villosa Roth var.glabrescens

作　　者：宗亚倩 吴晓祥 李荣[1] 马庆兵 段新慧[1] 姜华[1] ZONG Yaqian;WU Xiaoxiang;LI Rong;MA Qingbing;DUAN Xinhui;JIANG Hua(College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Yunnan Grassland and Feed Management Station,Kunming 650225,China;Administration and Protection Bureau of Black-necked Crane Provincial Reserve in Xundian County,Yunnan Province,Xundian 655200,China)

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南省草山饲料工作站,云南昆明650225 [3]云南寻甸黑颈鹤省级自然保护区管护局,云南寻甸655200

出　　处：《云南农业大学学报（自然科学版）》2021年第1期169-172,共4页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省重点研发计划项目(2018BB002-02);云南省技术创新人才(2018HB075)。

摘　　要：【目的】光叶紫花苕(Vicia villosa Roth var.glabrescens)是南方种植较为广泛的豆科绿肥作物。对光叶紫花苕的染色体核型进行分析,从细胞水平上研究其染色体特征,为揭示野豌豆属的染色体进化规律提供一定的理论参考。【方法】采用根尖染色体制片法,对供试材料根尖细胞有丝分裂中期的染色体进行核型分析和比较。【结果】光叶紫花苕的染色体相对长度组成为2n=14=4L+4M2+2M1+4S,数目为2n=2x=14,核型公式为2n=14=14m,核型不对称系数为55.9%,染色体类型为“1B”型。【结论】光叶紫花苕臂比指数主要集中在中部着丝粒m,属于进化上较为原始的种类。[Purpose]Vicia villosa Roth var.glabrescens is a leguminous green manure crop widely cultivated in the south of China.The analysis of the chromosome karyotype of V.villosa var.glabrescens and the study of chromosomal characteristics at the cellular level,can provide a certain theoretical reference for the chromosome evolution law of V.villosa var.glabrescens.[Method]The root-tip chromosome preparation method was used to analyze and compare the chromosomes of the mitotic chromosomes of the root-tip cells of the test material.[Result]V.villosa var.glabrescens chromosome relative length is composed of 2n=14=4L+4M2+2M1+4S.And the chromosome number was 2n=2x=14.Furthermore,the nuclear formula is 2n=14=14m,the nucleotype asymmetry coefficient is 55.9%and the chromosome type is:“1B”.[Conclusion]The arm ratio index of V.villosa var.glabrescens is mainly concentrated in the silk grains in the middle m,belongs to the primitive group in evolution.

关 键 词：光叶紫花苕 绿肥作物 染色体 核型分析 

分 类 号：S551.203[农业科学—作物学]