60Co γ射线辐射对裸鼹鼠肾脏、肺脏以及骨骼肌的影响  

Effects of Co γ-ray Radiation on Kidney, Lung and Skeletal Muscle Tissues of Naked Mole Rats

作　　者：张静远 张倩倩 赵懿宁[2] 杨蓉 张成财 贺辰囧 袁征 崔淑芳 ZHANG Jingyuan;ZHANG Qianqian;ZHAO Yining;YAGN Rong;ZHANG Chengcai;HE Chenjiong;YUAN Zheng;CUI Shufang(Department of Laboratory Animal Science,School of Basic Medical Sciences,Naval Medical University,Shanghai 200433,China;Department of Medical Technology,Yangpu District Mental Health Center,Shanghai 200090,China;Laboratory Animal Center,Academy of Military Medical Sciences,Beijing 100071,China)

机构地区：[1]海军军医大学基础医学院实验动物学教研室,上海200433 [2]上海市杨浦区精神卫生中心医技科,上海200090 [3]军事科学院军事医学研究院实验动物中心,北京100071

出　　处：《实验动物与比较医学》2020年第6期513-518,共6页Laboratory Animal and Comparative Medicine

基　　金：国家青年自然科学基金(31702075);上海市“科技创新行动计划”实验动物研究领域项目(20140900100);军队实验动物专项课题(SYDW[2016]009)。

摘　　要：目的观察60Coγ射线辐射后裸鼹鼠肾脏、肺脏和骨骼肌组织形态结构变化和相应组织氧化应激指标变化。方法裸鼹鼠经全身一次性10 Gy 60Coγ射线辐射,分别于辐射后7 d和14 d采集裸鼹鼠肾脏、肺脏和腓肠肌,制作石蜡切片,经HE染色后,光学显微镜下观察肾脏、肺脏和骨骼肌形态结构;采用动物组织生化检测试剂盒检测肾脏、肺脏和腓肠肌组织丙二醛(MDA)、总抗氧化能力(T-AOC)、谷胱甘肽(GSH)和氧化型谷胱甘肽(GSSG)等指标。结果辐射后裸鼹鼠肾脏、肺脏和骨骼肌均无明显病理改变。辐射后肾脏组织中的MDA含量显著升高(P <0.01),GSH含量先显著升高(P <0.05),后显著降低(P <0.05),GSSG和T-AOC未出现显著变化(P> 0.05);肺脏组织MDA含量显著升高(P <0.05),GSH、GSSG和T-AOC随时间延长先降低后升高(P <0.05);骨骼肌中MDA的含量显著上升(P<0.01),而GSH和GSSH的含量随时间的延长先降低后升高(P <0.01),T-AOC在辐射后7 d出现明显下降(P<0.01),14 d时仍然维持7 d水平。结论裸鼹鼠肾脏、肺脏和骨骼肌在高剂量60Coγ射线辐射后表现出一定程度的耐受能力,具体耐受机制有待深入研究。Objective To observe and analyze the changes of the kidney, lung, and skeletal muscle morphology and the related indicators of oxidative stress in naked mole rats after total body irradiation with 60Co γ-rays. Methods After 7 d and 14 d of total body radiation with 60Co γ-rays at a one-time dose of 10 Gy, the kidney, lung and gastrocnemius muscle samples of naked mole rats were collected.The microstructures of the samples were observed under an optical microscope after HE staining.Indicators such as malondialdehyde(MDA), total antioxidant capacity(T-AOC), glutathione(GSH)and oxidized glutathione(GSSG) were detected with the corresponding biochemical test kits.Results Microscopic examination showed that there were no obvious pathological changes in the kidney, lung and skeletal muscle tissues of the naked mole rats after the designed irradiation.Biochemical examination showed that the content of MDA in kidney increased significantly after radiation(P<0.01), the content of GSH was significantly increased at first and then declined(P<0.05),and the contents of GSSG and T-AOC did not changed dramatically(P>0.05). The content of MDA in lung was significantly increased(P<0.05), and the contents of GSH, GSSG and T-AOC decreased at first and then increased over time(P<0.05). The content of MDA in skeletal muscle was significantly increased(P<0.01), while the contents of GSH and GSSH decreased firstly and then increased over time(P<0.01). The content of T-AOC decreased significantly on the 7 th day after irradiation(P<0.01),and was maintained till the 14 th day. Conclusion The kidney, lung and skeletal muscle of naked mole rats showed a certain degree of tolerance to 60 Co γ-ray radiation, and the specific tolerance mechanism is worthy of further study.

关 键 词：裸鼹鼠 肾脏 肺脏 骨骼肌 60Coγ射线 

分 类 号：Q95-33[生物学—动物学]