60Co γ射线辐射对裸鼹鼠脾脏的影响  

Effect of Co γ-ray Radiation on Spleen of Naked Mole Rats

作　　者：杨蓉 赵懿宁[2] 杨文静 赵善民 柴宇静[2] 张成财 袁征 崔淑芳 YANG Rong;ZHAO Yining;YANG Wenjing;ZHAO Shanming;CHAI Yujing;ZHANG Chengcai;YUAN Zheng;CUI Shufang(Department of Laboratory Animal Science,School of Basic Medical Sciences,Naval Medical University,Shanghai 200433,China;Department of Medical Technology,Yangpu District Mental Health Center,Shanghai 200090,China;Laboratory Animal Center,Academy of Military Medical Sciences,Beijing 100071,China)

机构地区：[1]海军军医大学基础医学院实验动物学教研室,上海200433 [2]上海市杨浦区精神卫生中心医技科,上海200090 [3]军事科学院军事医学研究院实验动物中心,北京100071

出　　处：《实验动物与比较医学》2020年第6期519-522,527,共5页Laboratory Animal and Comparative Medicine

基　　金：国家青年自然科学基金(31700923);上海市“科技创新行动计划”实验动物研究领域项目(20140900100)。

摘　　要：目的观察60Coγ射线辐射后裸鼹鼠脾脏免疫系统及组织生物化学指标的变化,初步了解裸鼹鼠脾脏辐射耐受特性。方法用辐照剂量率为1.163 Gy/min,总剂量为10 Gy的60Coγ射线对裸鼹鼠全身一次性辐射。采集正常对照及辐照后7 d、14 d和21 d的裸鼹鼠脾脏,流式细胞术检测脾脏B淋巴细胞和巨噬细胞比例。采用动物组织生化检测试剂盒检测脾脏组织丙二醛(MDA)、总抗氧化能力(T-AOC)、谷胱甘肽(GSH)和氧化型谷胱甘肽(GSSG)等指标。结果流式细胞术检测结果显示,辐照后B淋巴细胞比例及巨噬细胞比例均明显升高(P<0.01)。生化检测结果显示,辐照后脾脏MDA水平明显升高(P <0.05);GSH和GSSH逐渐明显降低(P <0.05);辐照后T-AOC水平明显降低(P <0.01),但辐照后21 d有所上升,明显高于辐照后7 d和14 d(均P<0.01)。结论裸鼹鼠脾脏对60Coγ射线具有一定程度的耐受能力,但其耐受机制尚未明确。Objective To observe the changes of immune system and tissue biochemical indexes in the spleen of naked mole rats exposed to 60Co γ-ray radiation, for probing the radiation tolerance characteristics of the spleen of naked mole rats. Methods The whole body of naked mole rats was irradiated with a radiation dose rate of 1.163 Gy/min and a total dose of 10 Gy 60Co γ-rays. The spleens of the normal control and the exposed naked mole rats after 7 d, 14 d, and 21 d of irradiation were collected. The proportions of B lymphocytes and macrophages in the spleens were measured by flow cytometry. Malondialdehyde(MDA), total antioxidative capacity(T-AOC), glutathione(GSH)and oxidative glutathione(GSSG) in the spleen tissues were measured with corresponding biochemical assay kits. Results Flow cytometry results showed that the numbers of both B lymphocyte and macrophages increased significantly after irradiation(P<0.001). Biochemical examination results showed that the MDA level in spleen was significantly increased(P<0.05) after irradiation, the GSH and GSSH levels were significantly decreased(P<0.05), the T-AOC level was first significantly decreased(P<0.01)and then raised 21 days after irradiation, which was significantly higher than the indexes 7 days and 14 days after irradiation(P<0.01). Conclusion The spleens of naked mole rats can tolerate 60Co γ-ray radiation to some extent, but the mechanism of tolerance is not clear.

关 键 词：裸鼹鼠 辐射耐受性 脾脏 免疫 60Coγ射线 

分 类 号：Q95-33[生物学—动物学]