血府逐瘀汤提取过程中成分的量变规律研究  

Study on the quantitative change law of components in the extraction process of Xuefu Zhuyu Decoction

作　　者：薛梦洁 于卉娟 王璐瑶 柴欣 杨静 王跃飞 XUE Mengjie;YU Huijuan;WANG Luyao;CHAI Xin;YANG Jing;WANG Yuefei(Tianjin Key Laboratory of Traditional Chinese Medicine Chemistry and Analysis,Research Institute of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津中医药大学中医药研究院,天津市中药化学与分析重点实验室,天津301617

出　　处：《天津中医药》2021年第1期109-112,共4页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81873192)。

摘　　要：[目的]研究血府逐瘀汤提取过程中多种成分的量变规律。[方法]采用超高效液相色谱法和紫外分光光度法,研究血府逐瘀汤在不同提取时间和提取次数下水提液中各成分量变规律。[结果]随着提取时间的延长和提取次数的增加血府逐瘀汤的吸收光谱(200~500 nm)的吸光度值逐步增加并趋于稳定,主要成分苦杏仁苷、芍药苷、柚皮苷、新橙皮苷、甘草酸的提取率也呈现逐步增加并趋于稳定的趋势。[结论]血府逐瘀汤中主要化学成分的提取率与提取时间和次数呈正相关并随着提取时间和次数的增加逐步趋于稳定。[Objective]To study the quantitative change law of various components in the extraction process of Xuefu Zhuyu Decoction.[Methods]The quantitative changes of various components in the water extract of Xuefu Zhuyu Decoction at different extraction time and frequency were studied by ultra-high performance liquid chromatography(UPLC)and ultraviolet spectrophotometry.[Results]With the extension of extraction time and the increase of extraction frequency,the absorbance value of absorption spectrum(200~500 nm)of Xuefu Zhuyu Decoction gradually increased and tended to stabilize.The extraction rate of amygdalin,paeoniflorin,naringin,neohesperidin and glycyrrhizin acid also gradually increased and tended to stabilize.[Conclusion]The extraction rates of main chemical components in Xuefu Zhuyu Decoction are positively correlated with extraction time and frequency,and gradually tend to stabilize.

关 键 词：血府逐瘀汤 量变规律 紫外分光光度法 超高效液相色谱法 

分 类 号：R284[医药卫生—中药学]