碳点/β-环糊精复合物的制备及其用于尿酸检测的研究  

Preparation of carbon quantum dots/β-cyclodextrin complex and determination of uric acid

作　　者：李满秀[1] 曹艳蓉 任光明[1] 赵三虎[1] 李海平[1] LI Man-xiu;CAO Yan-rong;REN Guang-ming;ZHAO San-hu;LI Hai-ping(Department of Chemistry,Xinzhou Teachers’University,Xinzhou 034000)

机构地区：[1]忻州师范学院化学系,山西忻州034000

出　　处：《化学研究与应用》2021年第1期75-80,共6页Chemical Research and Application

基　　金：山西省1331工程重点学科建设计划项目(晋教财(2017)122)资助;材料与计算化学山西省高等学校重点实验室基金项目资助。

摘　　要：以柠檬酸三钠为碳源,采用微波辅助制备碳点,用β-环糊精(β-CD)和碳点复合制备出碳点/β-CD复合物,并用荧光、紫外、红外光谱等进行表征。在pH=6.6的磷酸盐缓冲溶液中,尿酸可使碳点/β-CD复合物的荧光增强,探讨了反应时间、反应温度、缓冲溶液及pH对荧光增强程度的影响,建立了测定尿样中尿酸含量的方法。碳点/β-CD复合物荧光增强程度与尿酸浓度在1.0×10^-6 mol·L^-1~3.0×10^-5 mol·L^-1范围内有良好的线性关系,回归方程为ΔF=4.8517 c+0.0106,相关系数r=1.00,检出限为4.7×10^-7 mol·L^-1。将该方法用于测定尿样中尿酸的含量,得到了令人满意的结果。Thesodium citratewere used as carbon source and microwave assisted to prepare carbon quantum dots.Then,combining it withβ-cyclodextrin obtain the carbon quantum dots/β-cyclodextrin(CQDs/β-CD)complex,The carbon quantum dots and CQDs/β-CD complexby fluorescence spectrum and UV spectrum,infrared spectrum were characterized in pH=6.6 phosphate buffer solution.uric acid can cause the CQDs/β-CD complex fluorescence enhancement,the effects of reaction time,reaction temperature,buffer solution and pH on the increasing amount offluorescence intensity were investigated,a new method of determination of uric acid in human urine was established accordingly,The linear relationship between increasing amount of fluorescence intensity(ΔF)and concentration of uric acid was kept in the range of 1.0×10^-6 mol·L^-1~3.0×10^-5 mol·L^-1,a correlation coefficient of 1.00,and a detection limit of 4.7×10^-7 mol·L^-1.Utilizing this to determine the contents of uric acid in human urine gets satisfactory results.

关 键 词：碳点/β-CD复合物 荧光增强 尿酸 

分 类 号：O657.3[理学—分析化学]