高通量测序分析5例进口鱼粉鱼源性成分构成  

Analysis of Fish-derived Components of Five Imported Fishmeal Samples via High Throughput Sequencing Method

作　　者：袁向芬[1] 吴绍强[1] 林祥梅[1] 吕继洲[1] YUAN Xiangfen;WU Shaoqiang;LIN Xiangmei;LYU Jizhou(Chinese Academy of Inspection and Quarantine,Beijing 100176,China)

机构地区：[1]中国检验检疫科学研究院,北京100176

出　　处：《中国畜牧兽医》2021年第1期93-100,共8页China Animal Husbandry & Veterinary Medicine

基　　金：国家重点研发计划课题(2017YFF0210302)。

摘　　要：为揭示鱼粉原料构成,保证鱼粉质量,本研究应用高通量测序技术对来源于不同国家的5例进口鱼粉样品进行测序,并基于COI基因及其丰度对鱼粉鱼源性成分构成进行分析。提取鱼粉样品DNA后应用鱼类通用COI基因扩增引物进行PCR扩增,得到鱼类混合COI基因序列产物,将扩增产物送测序公司进行高通量测序,将COI基因测序数据与鱼类相关数据库进行比对,通过比对结果进行鱼粉的物种源性成分分析。结果显示,成功获得了5例鱼粉样品中原料鱼成分,其中白鱼粉原料鱼主要成分为鳕鱼;而红鱼粉原料鱼成分较为多样,涉及鲱鱼、竹刀鱼等。通过Python对数据进行统计分析,绘制PCR扩增后鱼类COI基因丰度图,进一步直观提示鱼粉样品中原料的成分构成。本研究结果表明,高通量测序技术可应用于鱼粉质量评估,有助于快速了解鱼粉构成,对其质量及用途进行指导,同时,提示该技术可进一步应用于鱼粉掺杂掺假及致病微生物的监测工作中,对保障鱼粉产品质量、维护正常市场秩序具有重要意义。In order to reveal the composition of fishmeal and ensure the quality of fishmeal,high-throughput sequencing technology was used to sequence 5 imported fishmeal samples from different countries,and the composition of fishmeal derived from fish was analyzed based on COI gene and its abundance.After DNA was extracted from fishmeal samples,fish COI gene amplification primers were used for PCR amplification to obtain fish mixed COI gene sequence products.The amplified products were sent to the sequencing company for high-throughput sequencing.The COI gene sequencing data were compared with the fish related databases,and the species-derived components of fishmeal were analyzed through the comparison results.The results showed that 5 fishmeal samples were successfully obtained,and the main component of white fishmeal was cod.The composition of red fishmeal was diverse,including herring,swordfish and so on.The data were statistically analyzed by Python,and the COI gene abundance map of fish after PCR amplification was drawn to further intuitively indicate the composition of raw materials in fishmeal samples.The results showed that high-throughput sequencing technology could be used in the quality evaluation of fishmeal,which was helpful to quickly understand the composition of fishmeal and guide its quality and use.At the same time,it was suggested that this technology could be further applied to the monitoring of fishmeal adulteration and pathogenic microorganisms,which was of great significance to ensure the quality of fishmeal products and maintain the normal market order.

关 键 词：高通量测序 鱼粉 成分分析 COI基因丰度 

分 类 号：S816[农业科学—饲料科学]