反相高效液相色谱法测定人血清中泊沙康唑的浓度  

Determination of the concentration of posaconazole in human serum by reversed-phase high performance liquid chromatography

作　　者：计建军[1] 张慧[1] 查丽 费爱丽[1] 王小琴[1] 蔡继明[2] 徐少毅[2] 王长江[1] JI Jianjun;ZHANG Hui;ZHA Li;FEI Ai-li;WANG Xiao-qin;CAI Ji-ming;XU Shao-yi;WANG Changjiang(Department of Pharmacy,the Second Hospital Affiliated to Jiaxing University,Jiaxing 314000,China;Department of Critical Care,the Second Hospital Affiliated to Jiaxing University,Jiaxing 314000,China)

机构地区：[1]嘉兴学院附属第二医院药学部,浙江嘉兴314000 [2]嘉兴学院附属第二医院重症医学科,浙江嘉兴314000

出　　处：《中国药物应用与监测》2020年第6期367-370,共4页Chinese Journal of Drug Application and Monitoring

基　　金：嘉兴市公益性研究计划项目(2019AD32117)。

摘　　要：目的:建立RP-HPLC法测定人血清中泊沙康唑的浓度。方法:采用乙腈沉淀蛋白法对血样进行预处理,色谱条件为Agilent ZORBAX SB-C18分析柱(4.6 mm×250 mm,5μm),流动相为乙腈∶水(60∶40),检测波长为262 nm,流速1.0m L·min^-1,柱温25℃,内标为阿普唑仑。结果:泊沙康唑的线性范围为0.125~50.000μg·m L^-1(r=0.9996),方法回收率为99.80%,日间和日内RSD均<4%,绝对回收率为94.58%。对照品血清在室温放置24 h和–20℃冰箱保存10 d的稳定性RSD均<3%。测定患者血清中泊沙康唑药物浓度为1.18μg·m L-1。结论:本方法快速、准确、可靠,适用于泊沙康唑血药浓度的检测。Objective:To establish a method for determining the concentration of posaconazole in human serum by reversed-phase high performance liquid chromatography(RP-HPLC).Methods:The blood samples were pretreated by acetonitrile precipitation.The chromatographic column was Agilent ZORBAX SB-C18 analytical column(4.6 mm×250 mm,5μm).The mobile phase was acetonitrile and water(60∶40).The detection wavelength was 262 nm.The flow rate was 1.0 mL·min^-1.The column temperature was set as 25℃.And the internal standard was alprazolam.Results:The linear ranges of posaconazole were 0.125–50.000μg·mL^-1(r=0.9996),and the method recovery rates were 99.80%.The intra-day and inter-day relative standard deviations(RSD)were all less than 4%.The absolute recovery rates were 94.58%.The stability RSDs of control serum placed at room temperature for 24 h and stored in the refrigerator at–20℃for 10 day were all less than 3%.The serum concentration of posaconazole of the patient determined by this method was 1.18μg·mL-1.Conclusion:The method was fast,accurate and reliable,which was suitable for determining the blood concentration of posaconazole.

关 键 词：泊沙康唑 血药浓度 高效液相色谱法 

分 类 号：R978.5[医药卫生—药品]