氯虫苯甲酰胺对亚洲玉米螟解毒酶活性及生长发育的影响  

Effects of chlorantraniliprole on activities of detoxification enzymes and growth and reproduction of Ostrinia furnacalis

作　　者：支昊宇 丁新华[2] 陈萍 叶茂林 闫阳阳 姜卫华[1] ZHI Haoyu;DING Xinhua;CHEN Ping;YE Maolin;YAN Yangyang;JIANG Weihua(College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China;Institute of Plant Protection/Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis,Ministry of Agriculture and Rural Affairs,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China)

机构地区：[1]南京农业大学植物保护学院,江苏南京210095 [2]新疆农业科学院植物保护研究所/农业农村部西北荒漠绿洲作物有害生物综合治理重点实验室,新疆乌鲁木齐830091

出　　处：《南京农业大学学报》2021年第1期89-96,共8页Journal of Nanjing Agricultural University

基　　金：国家重点研发计划项目(2017YFD0201800)。

摘　　要：[目的]本文旨在明确新疆亚洲玉米螟田间种群对氯虫苯甲酰胺的敏感性及该药剂对玉米螟3种解毒酶活性及生长发育的影响。[方法]采用点滴法测定新疆乌鲁木齐、伊宁、疏勒和泽普4个田间种群对氯虫苯甲酰胺的敏感性,利用氯虫苯甲酰胺LD 20和LD 50处理玉米螟3龄幼虫72 h以及LD 50连续筛选9代,然后测定不同处理及不同田间种群的解毒酶活性并观察处理后的玉米螟生长发育状况。[结果]与乌鲁木齐种群相比,疏勒和泽普种群对氯虫苯甲酰胺的敏感性降低(抗性倍数分别为4.0和4.2倍)。伊宁种群的细胞色素P450酶和疏勒种群的以DCNB为底物的谷胱甘肽S-转移酶(GST)活性最高;氯虫苯甲酰胺LD 20和LD 50处理玉米螟72 h可导致其GST(DCNB为底物)活性显著升高,分别是对照的1.46和1.86倍;连续用氯虫苯甲酰胺LD 50筛选的玉米螟F5和F8代的P450酶活性是敏感品系的4.27和5.09倍,F5和F8代的GST(底物CNDB)活性以及F8代GST(底物DCNB)活性是敏感品系的1.27、1.33和1.36倍。LD 20处理72 h可使当代种群蛹重和单雌产卵量显著降低,连续用氯虫苯甲酰胺LD 50处理玉米螟9代可导致F10代幼虫期延长,蛹重和成虫羽化率增加,而单雌产卵量及孵化率比对照降低14.83%和5.84%,最终选育品系的内禀增长率显著低于敏感品系。[结论]新疆地区玉米螟对氯虫苯甲酰胺抗性产生的风险较高,氯虫苯甲酰胺对P450和GST有诱导作用,这2种代谢酶可能参与玉米螟对氯虫苯甲酰胺抗性的形成。[Objectives]The study aimed to understand the resistance status of Ostrinia furnacalis populations from Xinjiang to chlorantraniliprole(CAP)and effects of Asian corn borer treated by chlorantraniliprole on the activities of three detoxifying enzymes,including esterase(EST),glutathione S-transferase(GST)and cytochrome P450 enzyme(P450),and population breeding.[Methods]The susceptibilities to chlorantraniliprole of the populations collected from Urumqi,Yining,Shule and Zepu in Xinjiang,were determined using topical application.3rd instar larvae of O.furnacalis were treated with LD 20 and LD 50 dose of chlorantraniliprole for 72 h and selected continuously for 9 generations with LD 50 dose of chlorantraniliprole(CAP-Sel).The activities of detoxification enzymatic were analyzed by in vitro enzyme activity assay in different treatments and populations of O.furnacalis and the development of tested insects was observed.[Results]Compared with the relative susceptible population Urumqi,Shule and Zepu populations had decreased sensitivity to chlorantraniliprole with resistance ratio of 4.0-and 4.2-folds,respectively.The highest activity of P450 was detected in Yining population,while GST(DCNB as substrate)activity of Shule population was the highest among the tested populations.The activities of GST(DCNB as substrate)in the LD 20 and LD 50 treatment groups for 72 h increased significantly by 1.46-and 1.86-folds compared with the control respectively.Furthermore,the activities of the three detoxification enzymes in CAP-Sel strain were promoted along with the increase of generations and the change of P450 activities were the most significant,which in the F5 and F8 generations were enhanced by 4.27-and 5.09-folds,meanwhile,the activities of GST(CDNB as substrate)in F5 and F8 generations and GST(DCNB as substrate)in the F8 generation were increased by 1.27-.1.33-and 1.36-folds compared to those in susceptible strain,respectively.The sublethal dose(LD 20)of chlorantraniliprole for 72 h significantly lowered the weight of pupae

关 键 词：亚洲玉米螟 氯虫苯甲酰胺 解毒酶 生长和发育 亚致死效应 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治]