抗氧化合剂对膝骨关节炎模型大鼠结构性退变的作用  

Effect of antioxidant mixture on structural degeneration of an osteoarthritis rat model

作　　者：罗安玉[1] 刘瀚霖[1] 谢小飞 黄琛 Luo Anyu;Liu Hanlin;Xie Xiaofei;Huang Chen(Department of Orthopedics,Hanyang Hospital,Wuhan University of Science and Technology,Wuhan 430050,Hubei Province,China)

机构地区：[1]武汉科技大学附属汉阳医院骨二科,湖北省武汉市430050

出　　处：《中国组织工程研究》2021年第23期3625-3629,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:氧化应激在骨关节炎的病理过程中起重要作用,导致关节局部炎症和基质降解。此前的研究表明,槲皮素和维生素C等抗氧化剂是治疗骨关节炎的潜在候选药物。目的:确定槲皮素、维生素C以及甲磺酸去铁胺合剂是否可以延缓创伤后膝骨关节炎大鼠模型的疾病进展。方法:切断10只SD大鼠的前交叉韧带,构建膝骨关节炎动物模型,随机分为2组,每组5只。术后20周,将50μL抗氧化合剂(治疗组)或生理盐水(对照组)缓慢注入大鼠术侧膝关节,每周1次,连续4周。抗氧化合剂包含30 g/L维生素C、1 g/L二羟槲皮素和200 mg/L甲磺酸去铁胺。在治疗前、治疗后1周和5周进行步态分析;治疗后5周进行显微CT检测和组织学评分。结果与结论:①治疗组大鼠步态分析显示,关节腔注射抗氧化合剂不会随着时间的推移改善疼痛相关肢体怠惰指数(P=0.449);然而在治疗后5周,治疗组的肢体怠惰指数显著低于对照组(P=0.047);②治疗后5周,显微计算机断层扫描分析显示,两组大鼠的任何参数差异均无显著性意义(P>0.05);③两组动物均存在严重的膝骨关节炎组织病理学改变;两组国际骨关节炎研究学会评分相比,差异无显著性意义(P=0.382);④提示关节腔注射含有槲皮素、维生素C和去铁胺的抗氧化剂合剂不会延缓晚期膝骨关节炎的进展;未来的研究应关注在早期膝骨关节炎中给予抗氧化剂,并延长药物滞留时间,观察其是否能有效延缓膝骨关节炎的进展。BACKGROUND:Oxidative stress plays an important role in the pathological process of osteoarthritis,leading to local inflammation and matrix degradation.Previous studies have shown that antioxidants such as quercetin and vitamin C are potential candidates for treating osteoarthritis.OBJECTIVE:To determine whether quercetin,vitamin C and desferrioxamine mesylate mixture can delay disease progression in rats with post-traumatic osteoarthritis.METHODS:The anterior cruciate ligament was cut off in 10 Sprague-Dawley rats to induce osteoarthritis.Animal models were randomized into two groups(n=5per group).Twenty weeks after operation,50μL of normal saline or antioxidant mixture was slowly injected into the affected joint,once a week for 4 successive weeks.The antioxidant mixture consisted of 1 g/L dihydroxyquercetin,30 g/L vitamin C and 200 mg/L desferrioxamine mesylate.Gait analysis was performed before treatment,1 week and 5 weeks after treatment.Micro-CT examination and histology scoring were performed 5 weeks after treatment.RESULTS AND CONCLUSION:Gait analysis showed that intra-articular injection of antioxidant mixture did not improve pain-related limb laziness index(P=0.449).However,5 weeks after treatment,the limb laziness index in the treatment group was significantly lower than that in the control group(P=0.047).Five weeks after treatment,microcomputed tomography analysis showed that there was no significant difference in any parameters between the treatment group and the control group(P>0.05).There were severe histopathological changes of osteoarthritis in both groups.The Osteoarthritis Research Society International scores showed no significant difference in both groups(P=0.382).In conclusion,intra-articular injection of antioxidant mixture containing quercetin,vitamin C and desferrioxamine does not delay the progress of osteoarthritis in patients with advanced osteoarthritis.Future studies should aim to determine whether administration of antioxidants and prolonged drug retention time in early osteoarthriti

关 键 词：骨 膝关节 抗氧化剂 骨关节炎 氧化应激 槲皮素 维生素C 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]