肾衰饮调控慢性肾功能衰竭大鼠肾脏细胞凋亡的机制  

Regulatory mechanism of Shenshuai Yin on cell apoptosis in the kidney of chronic renal failure rats

作　　者：张亮[1,2] 马晓燕[2] 王佳虹 Zhang Liang;Ma Xiaoyan;Wang Jiahong(Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning Province,China;Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning Province,China;Shenyang Pharmaceutical University,Shenyang 110015,Liaoning Province,China)

机构地区：[1]辽宁中医药大学,辽宁省沈阳市110032 [2]辽宁中医药大学附属医院,辽宁省沈阳市110032 [3]沈阳药科大学,辽宁省沈阳市110015

出　　处：《中国组织工程研究》2021年第23期3672-3677,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81803769),项目负责人:王佳虹。

摘　　要：背景:前期动物实验已经做过肾衰饮治疗慢性肾功能衰竭在内质网凋亡通路方面的研究,但其有关细胞凋亡其他途径的机制还有待研究。目的:从Fas/FasL途径探讨肾衰饮对慢性肾功能衰竭大鼠肾脏细胞凋亡的调控机制。方法:选取60只雄性SD大鼠,随机分成空白组、模型组、尿毒清组和肾衰饮组,后3组通过腺嘌呤悬浊液200 mg/(kg•d)灌胃建立慢性肾功能衰竭大鼠模型;肾衰饮组大鼠给予肾衰饮煎剂33.3 g/(kg•d)灌胃;尿毒清组大鼠给予尿毒清颗粒2.25 g/(kg•d)灌胃;空白组和模型组给予0.9%氯化钠注射液8 mL/kg灌胃;均为1次/d,疗程4周。治疗完成后检测血清肌酐、尿素氮水平;苏木精-伊红染色法观察大鼠肾脏组织病理学变化,原位末端标记法染色评价肾组织细胞凋亡情况;免疫印迹法检测大鼠肾脏组织Fas、FasL、Caspase8、Caspase3蛋白表达水平。实验方案经辽宁中医药大学实验动物伦理委员会批准(批准号为21000092017044)。结果与结论:①与空白组比较,模型组、尿毒清组和肾衰饮组大鼠的血清肌酐、尿素氮水平显著升高(P<0.01);肾脏细胞凋亡指数明显升高(P<0.01);肾脏组织中Fas、FasL、Caspase3、Caspase8蛋白表达水平均明显升高(P<0.01);尿毒清组和肾衰饮组大鼠以上指标均优于模型组(P<0.01),尿毒清组和肾衰饮组相比差异无显著性意义(P>0.05);②结果说明,肾衰饮能够降低慢性肾功能衰竭大鼠肾脏细胞凋亡,改善肾脏功能,其机制可能与肾衰饮能够抑制Fas/FasL介导的凋亡途径相关。BACKGROUND:Previous studies in animals have explored the pathway of apoptosis in the endoplasmic reticulum after treatment with Shenshuai Yin for chronic renal failure.However,the mechanism of cell apoptosis through other pathways remains to be studied.OBJECTIVE:To investigate the regulatory mechanism of renal cell apoptosis of chronic renal failure rats through Fas/FasL pathway.METHODS:Sixty male Sprague-Dawley rats were randomly divided into a blank group,a model group,a Niaoduqing group and a Shenshuai Yin group.A rat model of chronic renal failure was established by intragastric administration of adenine suspension 200 mg/kg per day.Rats in the Shenshuai Yin group were given Shenshuai Yin 33.3 g/kg per day by gavage.Rats in the Niaoduqing group were given Niaoduqing granule 2.25 g/kg per day by gavage.Rats in the blank group and model group were given 0.9%sodium chloride injection 8 mL/kg by gavage.Treatments in each group were conducted once a day for 4 consecutive weeks.After treatment,serum creatinine and urea nitrogen levels were detected.Renal histopathological changes were observed by hematoxylin-eosin staining.Apoptosis in renal tissues was evaluated by TUNEL staining.The protein levels of Fas,FasL,Caspase8 and Caspase3 were detected by western blot.An approval by the Animal Ethics Committee of Liaoning University of Traditional Chinese Medicine was given with an approval No.21000092017044.RESULTS AND CONCLUSION:Compared to the blank group,the levels of serum creatinine and urea nitrogen were significantly increased in the model,Niaoduqing and Shenshuai Yin groups(P<0.01),the apoptotic index of renal cells was significantly increased(P<0.01),and the protein levels of Fas,FasL,Caspase3 and Caspase8 were significantly increased(P<0.01).The above indexes of Niaoduqing group and Shenshuai Yin group were all significantly improved as compared to the model group(P<0.01),but there was no significant difference between the Niaoduqing group and Shenshuai Yin group(P>0.05).To conclude,Shenshuai Yin can reduce re

关 键 词：慢性肾功能衰竭 肾衰饮 细胞凋亡 FAS/FASL 大鼠 模型 动物 

分 类 号：R446[医药卫生—诊断学] R496[医药卫生—临床医学]