急性钳夹型脊髓损伤模型大鼠造模方式的选择:一项网状Meta分析  

Selection of modeling methods for acute compressive spinal cord injury:a network Meta-analysis

作　　者：徐伟龙 左媛[2] 辛大奇[2] 贺晨阳 赵鹏[1] 史鸣 周博源 刘雅婷 赵岩[2] Xu Weilong;Zuo Yuan;Xin Daqi;He Chenyang;Zhao Peng;Shi Ming;Zhou Boyuan;Liu Yating;Zhao Yan(Inner Mongolia Medical University,Hohhot 010000,Inner Mongolia Autonomous Region,China;The Second Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010000,Inner Mongolia Autonomous Region,China)

机构地区：[1]内蒙古医科大学,内蒙古自治区呼和浩特市010000 [2]内蒙古医科大学第二附属医院,内蒙古自治区呼和浩特市010000

出　　处：《中国组织工程研究》2021年第23期3767-3772,共6页Chinese Journal of Tissue Engineering Research

基　　金：内蒙古自治区科技计划项目(2019GG157),项目负责人:左媛。

摘　　要：目的:用动脉瘤夹创建急性钳夹型脊髓损伤模型是一种简单有效的造模方法。然而,为了建立这种急性钳夹型脊髓损伤动物模型,许多实验根据不同闭合力的动脉瘤夹和压迫时间采用了不同的造模方式,其中包括30 g压迫力的动脉瘤夹压迫脊髓60 s(30 g,60 s组);70 g压迫力的动脉瘤夹压迫脊髓30 s(70 g,30 s组);70 g压迫力的动脉瘤夹压迫脊髓60 s(70 g,60 s组);动脉瘤夹压迫脊髓至其直径的1/2,压迫时间为30 s(1/2,30 s组)。文章的目的是探讨这4种造模方式是否存在显著性差异,并且找出最适合创建急性钳夹型脊髓损伤模型的方法。方法:计算机检索PubMed、EMbase、中国知网、维普和万方数据库等数据库截止2019-10-01公开发表的关于急性脊髓损伤大鼠造模的文献。依据设定的纳入排除标准对文献进行筛选,并对纳入的文献进行数据提取和质量评价。最后运用RevMan软件和ADDIS软件,采用Meta分析、亚组分析和网状Meta分析的方法评价不同建模方法对脊髓损伤大鼠肢体功能的影响。结果:①共纳入14篇文献质量评价为6-8分的随机对照动物实验,共包含714只SD大鼠;②Meta分析结果显示,术后第1周,各造模方式的BBB评分排序结果依次为:假手术组>30 g,60 s组>1/2,30 s组>70 g,60 s组>70 g,30 s组,其中用30 g闭合力的动脉瘤夹压迫脊髓60 s组在术后第1周的BBB评分最高,明显高于其他3组(P<0.05);③亚组分析结果提示,急性脊髓损伤的运动恢复与大鼠性别有关,大鼠昼夜活动的差异影响BBB评分的测定。结论:文章阐述的4种造模方法都可构建急性钳夹型脊髓损伤动物模型,以运动功能恢复方面为判断标准时,用30 g闭合力的动脉瘤夹压迫脊髓60 s是目前已发表文献所显示的最佳造模方法,同时建议使用雌性大鼠进行造模,但未来还需进一步研究验证。OBJECTIVE:Generating an acute compressive spinal cord injury model with an aneurysm clip is a simple and effective modeling method.However,to create the animal model of acute compressive spinal cord injury,many experimental studies have utilized diverse modeling methods according to different closure force aneurysm clips and duration,including aneurysm clipping with 30 g compression force to compress the spinal cord for 60 seconds(30 g,60 s);aneurysm clipping with 70 g compression force to compress the spinal cord for 30 seconds(70 g,30 s);aneurysm clipping with 70 g force to compress the spinal cord for 60 seconds(70 g,60 s);compressing the spinal cord to 1/2 of its diameter for 30 seconds by aneurysm clips(1/2,30 s).The present study aimed to assess whether there is a significant difference among these modeling methods,and to determine the method that is most suitable to establish the clamp-type spinal cord injury model.METHODS:A literature search of PubMed,Embase,CNKI,VIP,and WanFang databases was conducted for relevant studies from their inception dates to October 1,2019.The included literatures were initially screened according to the inclusion and exclusion criteria,and data extraction and quality evaluation were performed subsequently.The paired Meta-analysis,subgroup analysis,and network Meta-analysis were conducted to evaluate the effects of different modeling methods on the limb function of spinal cord injury rats.RESULTS:Fourteen articles with a total of 714 Sprague-Dawley rats were included in the study.All the included articles were scored 6-8 for quality evaluation.The Basso,Beattie and Bresnahan(BBB)scores of the four modeling methods in the 1st week after modeling ranked as follows:sham operation group>30 g,60 s group>1/2,30 s group>70 g,60 s group>70 g,30 s group.The BBB score in the 30 g,60 s group was significantly higher than that in the 1/2,30 s group,70 g,60 s group and 70 g,30 s group at 1 week postoperatively(P<0.05).Subgroup analyses suggested an association of locomotor recovery of spina

关 键 词：脊髓损伤 模型 动脉瘤 大鼠 运动恢复 性别 网络Meta分析 循证医学 

分 类 号：R453[医药卫生—治疗学] R363[医药卫生—临床医学]