两株菲降解菌的降解特性及动力学  

Degradation Characteristics and Kinetics of Two Phenanthrene-Degrading Bacteria

作　　者：梁雪涛 苏俊杰 王震 杨琦[1] LIANG Xuetao;SU Junjie;WANG Zhen;YANG Qi(Beijing Key Laboratory of Water Resource&Environmental Engineering,China University of Geoscience〈Beijing〉,MOE Key Laboratory of Groundwater Circulation and Environmental Evolution,China University of Geoscience〈Beijing〉,Beijing 100083,China)

机构地区：[1]中国地质大学〈北京〉水资源与环境工程北京市重点实验室,中国地质大学〈北京〉地下水循环与环境演化教育部重点实验室,北京100083

出　　处：《净水技术》2020年第S02期40-48,共9页Water Purification Technology

基　　金：北京市产学研项目(51900265005);国家科技重大专项(2009ZX07207-008,2009ZX07419-002,2009ZX07207-001,2015ZX07406005-001);中央高校基本科研业务费专项资金(2652013101,2652013086,2652013087);国家重大科学仪器设备开发专项(2012YQ060115);大气污染成因与控制技术研究重点专项(2016YFC0209205)。

摘　　要：从实验室小型MBR池中,筛选纯化得到两株菲降解菌,对比研究不同环境因素对两种菌株降解菲的影响,并利用Monod动力学模型对结果进行拟合。研究发现:两株菲降解菌(菌株Na-B与Na-S)经鉴定后分别为短芽孢杆菌(Bacillus brevis)与鞘氨醇单胞菌(Sphingomonas sp.)。两株菌对菲有较好的降解效果,最佳环境条件:菌悬液投加量为20 mL,温度为28℃,pH值为7.2。在最佳环境条件下,菌株Na-B在48 h内对菲(1.0 mg/L)的降解率达98.8%,菌株Na-S在56 h内对菲(1.0 mg/L)的降解率可达96.3%。比较两菌株的综合特性,菌株Na-S更适应酸性环境,且具有较强的低温耐受性;菌株Na-B的酸碱耐受范围较大而温度适应性较差。两菌株对菲的降解用Monod动力学模型进行模拟,得到菌株Na-B与菌株Na-S对菲的最大比降解速度vmax与饱和常数K_S分别为1.158 mg/(L·h)、11.905 mg/L与0.951 mg/(L·h)、16.311 mg/L。Two phenanthrene-degrading strains were acclimated and isolated from membrane bioreactor in the laboratory,and effects of different reaction conditions on degradation efficiency of phenanthrene were evaluated.Monod kinetic model was used to fit the results.The study found that two phenanthrene-degrading strains(strains Na-B and Na-S)were identified as Bacillus brevis and Sphingomonas sp..The two strains had a good degradation effect on phenanthrene.The best reaction conditions were as follows:bacterial suspension was 20 mL,temperature was 28℃,and pH value was 7.2.Under the optimal reaction conditions,degradation rate of phenanthrene(1.0 mg/L)was 98.8%by strain Na-B within 48 hours,and 96.3%within 56 hours by strain Na-S.Compared comprehensive characteristics with the two strains,strain Na-S was more adaptable to acid environment and had stronger low-temperature tolerance,while strain Na-B had a wider range of pH value and poor temperature adaptability.The reaction was conformed to Monod kinetic,which showed the maximum specific substrate degradation rate vmax and saturation constant KS of strain Na-B and strain Na-S were 1.158 mg/(L·h),11.905 mg/L and 0.951 mg/(L·h),16.311 mg/L.

关 键 词：菲 生物降解 Monod动力学 

分 类 号：X522[环境科学与工程—环境工程]