萨梅诺娃新佩雷斯虫中国新记录  

NEW RECORD OF NEOPEREZIA SEMENOVAIAE ISSI,ET AL.2012 INFECTING THE FAT BODY OF CHIRONOMUS PLUMOSUS LARVA IN CHINA

作　　者：刘新华 翁美其[2,3] 宋锐 赵媛莉[2,3] 章晋勇[2,3,4] 肖调义 LIU Xin-Hua;WEN Mei-Qi;SONG Rui;ZHAO Yuan-Li;ZHANG Jin-Yong;XIAO Tiao-Yi(Laboratory of Hydrobiology,Hunan Agricultural University,Changsha 410128,China;Huai’an Research Center,Key Laboratory of Aquaculture Diseases Control,Ministry of Agriculture and Rural Affairs,State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China;University of Chinese Academy of Sciences,Beijing 100049,China;School of Marine Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China;Hunan Fisheries Science Institute,Changsha 410153,China)

机构地区：[1]湖南农业大学动物科学技术学院,长沙410128 [2]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,农业农村部淡水养殖病害防治重点实验室,淮安研究中心,武汉430072 [3]中国科学院大学,北京100049 [4]青岛农业大学海洋科学与工程学院,青岛266109 [5]湖南省水产科学研究所,长沙410153

出　　处：《水生生物学报》2021年第1期140-145,共6页Acta Hydrobiologica Sinica

基　　金：湖南省现代农业产业技术体系项目(湘农发[2019]05号);湖南农业大学神农学者青年英才人才引进经费(540741900599);国家自然科学基金(31772411和31972787);中国科学院水生生物研究所自主探索课题;湖北省自然科学基金(2018CFA074);山东省泰山学者青年专家(tsqn201909133);青岛农业大学高层次人才引进经费资助。

摘　　要：研究报道了中国首例摇蚊微孢子虫,结合各发育阶段形态特征、生态学特征及分子特征,鉴定其为萨梅诺娃新佩雷斯虫Neoperezia semenovaiae Issi,et al.2012,系我国新记录。萨梅诺娃新佩雷斯虫寄生于羽摇蚊幼虫脂肪体组织,导致其体表呈白浊状。成熟孢子呈卵圆形,孢子长(5.7±0.2)μm(5.3—6.3μm),宽(3.7±0.1)μm(3.4—4.0μm)。透射电镜观察显示各发育阶段均为离核,发育不同步,与宿主细胞质直接接触。早期发育阶段为高电子密度的多核裂殖体阶段,经原生质团分裂形成单核或多核产孢体,进一步发育为单核孢子母细胞。孢子母细胞形状不规则,周围被内质网环绕,并逐渐形成微孢子虫的典型结构如极丝、极质体和三层孢壁等。成熟孢子卵圆形,离核,细胞核较大,位于孢子正中央,被大量核糖体包围。极质体分为两部分,前半部分为海绵状,后半部分薄膜状。锚状盘位于孢子前端,呈蘑菇状。孢壁三层,外层为高电子密度层,厚26.5—62.7 nm,中间层为电子透明层,厚151.8—236.1 nm,里层为质膜层。同型极丝,30—31圈,分2—3列排列。扩增获得其小核糖体序列为1356 bp,序列比较发现其与俄罗斯列宁格勒区羽摇蚊的N.semenovaiae相似性为99.1%。系统发育关系分析表明N.semonovaiae与Neoperezia、Bryonosema、Schroedera属种类聚为一独立进化枝,N.semonovaiae种群出现明显的地理分化。Based on its ultrastructural features of different developmental stages,molecular characteristics,and ecological considerations,the present study firstly discovered and identified Neoperezia semenovaiae Issi,et al.2012 infecting the fat body of Chironomus plumosus larva in China.Transmission electron microscopy revealed numerous life stages of this microsporidian parasite.All developmental stages were monokaryotic and were in direct contact with the host cell cytoplasm.The early development stages observed were multinucleate merogonial plasmodia with high electron density.Later,merogonial plasmodia underwent plasmotomy to the formation of uninucleate or binucleate sporonts which further developed into sporoblasts.Sporoblasts were irregular in shape,surrounded by endoplasmic reticulum and transformed into mature spores.Mature spores were oval and monokaryotic,with a length of(5.7±0.2)μm(5.3—6.3μm)and a width of(3.7±0.1)μm(3.4—4.0μm).Spores possessed a bipartite polaroplast composed of an anterior spongy region and posterior lamellar membrane.Spore wall is trilaminar including an electron dense exospore measuring 26.5—62.7 nm,an electron lucent endospore with a thick of 151.8—236.1 nm,and a thin plasma membrane.Polar filaments were isofilar,coiled with 31—33 turns and arranged in 2 or 3 rows.The obtained sequences from amplification of the partial SSU rDNA was 1356 bp in length,which is 99.1%similarity to N.semenovaiae.Phylogenetic analysis based on the aligned partial SSU rDNA sequences indicated that N.semenovaiae clustered with species of Neoperezia,Bryonosema and Schroedera and this microsporidian parasite had undergone obvious genetic geographical differentiation.

关 键 词：水生微孢子虫 摇蚊幼虫 萨梅诺娃新佩雷斯虫 分类学 新记录 

分 类 号：Q959.11[生物学—动物学]