单细胞测序在心血管系统中的应用  

Application of Single Cell Sequencing in Cardiovascular System

作　　者：潘乐[1] 汪翔[1] 龚惠[1] PAN Le;WANG Xiang;GONG Hui(Shanghai Institute of Cardiovascular Diseases,Zhongshan Hospital,Institutes of Biomedical Sciences,Fudan University,Shanghai 200032,China)

机构地区：[1]上海市中山医院心血管病研究所复旦大学生物医学研究所,上海200030

出　　处：《心血管病学进展》2020年第12期1227-1230,1267,共5页Advances in Cardiovascular Diseases

基　　金：国家自然科学基金面上项目(81770239)。

摘　　要：近十年,高通量测序技术高速发展,特别是在单细胞转录组学领域。单细胞测序是从单个细胞的水平检测基因表达信息,从而可以揭示那些原本在组织样本测序中被掩盖的信息,这一新兴测序技术在各个领域都有着广阔的应用前景,特别是在肿瘤、免疫等领域,是近年来迅速兴起的一项技术。单细胞测序可以区分不同的细胞类型及其亚型,了解其基因表达,这一新兴技术在心脏发育和疾病模型的探索中已得到广泛的应用。现就聚焦单细胞测序技术的发展及其在心血管领域的应用前景做一综述。In the last decade,high-throughput sequencing technologies have developed rapidly,especially in the field of single-cell transcriptomics.Single-cell RNA sequencing(scRNA-seq)detects gene expression information at the level of a single cell,thus revealing information that would otherwise be obscured in tissue sample sequencing.This new sequencing technology has a good application prospect in various fields,especially in tumor,immunity and other fields.It has developed rapidly in recent years.ScRNA-seq can distinguish between different cell types and their subtypes to understand their gene expression,thus,this emerging technology has been widely used in the exploration of models of heart development and disease.This article focus on the development of single-cell transcriptomics and its application in the cardiovascular field.

关 键 词：单细胞RNA测序 心脏细胞 心血管疾病 

分 类 号：R54[医药卫生—心血管疾病]