氟西汀对抑郁症模型小鼠Treg细胞的影响  

Effect of fluoxetine on Treg cell subsets in depression mice

作　　者：马博乙 张静[1] 吴玉婷 张博文 曹雅明[1] 李成(指导)[1] MA Bo-Yi;ZHANG Jing;WU Yu-Ting;ZHANG Bo-Wen;CAO Ya-Ming;LI Cheng(Immunology Department of China Medical University,Shenyang 110000,China)

机构地区：[1]中国医科大学免疫学教研室,沈阳110000

出　　处：《中国免疫学杂志》2020年第24期2956-2959,共4页Chinese Journal of Immunology

基　　金：2019年大学生创新创业训练计划资助项目(201910159054)。

摘　　要：目的:探讨CUMS抑郁症模型小鼠经氟西汀治疗后Treg细胞的改变。方法:采用慢性不可预知温和应激(CUMS)建立抑郁小鼠模型,糖水偏好和摄食比作为抑郁行为学的评估指标。通过免疫组化、流式细胞术检测抑郁小鼠中Treg细胞数的变化。结果:免疫组化结果显示CUMS模型组脾脏Treg细胞数量与对照组相比明显升高,经氟西汀治疗后Treg细胞数量下调(P<0.05);流式细胞术检测结果显示相比CUMS模型组及对照组氟西汀治疗后Treg细胞占CD4+T细胞明显降低。结论:CUMS模型小鼠抑郁症状可通过氟西汀治疗后改善,并伴随着Treg细胞亚群比例的降低。Objective:To explore the changes of Treg cells in CUMS depression model mice and after fluoxetine treatment.Methods:Chronic unpredictable mild stress(CUMS)was used to establish the model of depression mice,the preference of sugar water and the ratio of food consumption as the evaluation index of the behavior of depression.The changes of the number of Treg cells in depressed mice were detected by immunohistochemistry and flow cytometry.Results:The results of immunohistochemistry showed that the number of Treg cells in the spleen of the CUMS model group was significantly higher than that of the control group,and the number of Treg cells was down-regulated after fluoxetine treatment(P<0.05);the results of flow cytometry showed that compared with the CUMS model group and In the control group,the percentage of Treg cells in CD4+T cells decreased significantly after fluoxetine treatment.Conclusion:Depression symptoms in CUMS model mice can improved after fluoxetine treatment,accompanied by a decrease in the proportion of Treg cell subsets.

关 键 词：抑郁症 慢性不可预知温和应激 氟西汀 TREG细胞 

分 类 号：R392.1[医药卫生—免疫学]