黄花菜外植体初代培养比较及快繁技术研究  

Comparison of Primary Culture Using Different Explants of Hemerocalli citrine and Study of Rapid Propagation Technology

作　　者：蔡宣梅[1] 郭文杰[1] 张洁[1] 方少忠[1] CAI Xuan-mei;GUO Wen-jie;ZHANGJie;FANG Shao-zhong(Institute of Biotechnology,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China)

机构地区：[1]福建省农业科学院生物技术研究所,福建福州350003

出　　处：《东南园艺》2020年第5期21-25,共5页Southeast Horticulture

摘　　要：选用黄花菜新品种‘台中6号’的鳞茎、根尖、花梗、花丝、花蕾等5种外植体,进行愈伤组织的诱导及植株再生研究。结果表明:根尖污染率高,培养后褐化,死亡;花丝污染率低,可以诱导出愈伤组织,但继代后逐渐死亡;幼嫩花蕾可以诱导出愈伤组织,并分化成完整植株;花梗和鳞茎可以直接诱导出植株,因此,黄花菜组织培养适宜的外植体为花梗、花蕾和鳞茎。其快繁体系为:诱导培养基BA 0.5 mg·L-1+NAA 0.25 mg·L-1+2,4-D 0.5 mg·L-1、继代培养基BA 2.0 mg·L-1+NAA 0.1 mg·L-1、生根培养基1/2MS+BA 0.2 mg·L-1+NAA 0.05 mg·L-1。The bulb,root-tip,pedicel,filament and flower bud of Hemerocalli citrine were used as explants to study callus induction and regenerated plant.The results showed that contamination rate of the root tip was high and it became brown and died after culture.Contamination rate of filament was low,which could induce callus but gradually dies after subculture.The young flower bud could induce callus and differentiate into complete plants.That pedicels and bulbs could directly induce regenerated plants.So the suitable explants for tissue culture were bulb,pedicel,and flower bud.The rapid propagation system was as follows:the induction medium was MS+BA 0.5 mg·L^-1+NAA 0.25 mg·L^-1+2,4-D 0.5 mg·L^-1;the subculture medium was MS+BA 2.0 mg·L^-1+NAA 0.1 mg·L^-1;the rooting medium was 1/2MS+BA 0.2 mg·L^-1+NAA 0.05 mg·L^-1.

关 键 词：黄花菜 外植体 组织培养 快繁技术 

分 类 号：S644.3[农业科学—蔬菜学]