血清白蛋白-铜酞菁纳米粒子用于线粒体靶向光疗  

Fabrication of serum albumin-copper phthalocyanine nanoparticles for mitochondria-targeted phototherapy

作　　者：于富强 杜健军[1,2] 路杨 马贺 樊江莉 孙文[1,2] 龙飒然 彭孝军 YU Fuqiang;DU Jianjun;LU Yang;MA He;FAN Jiangli;SUN Wen;LONG Saran;PENG Xiaojun(State Key Laboratory of Fine Chemicals,Dalian University of Technology,Dalian 116024,Liaoning,China;Ningbo Research Institute,Dalian University of Technology,Ningbo 315016,Zhejiang,China)

机构地区：[1]大连理工大学精细化工国家重点实验室,辽宁大连116024 [2]大连理工大学宁波研究院,浙江宁波315016

出　　处：《化工学报》2021年第1期597-608,共12页CIESC Journal

基　　金：国家自然科学基金委创新群体项目(21421005);国家自然科学基金面上项目(21676047,21878039);国家自然科学基金优秀青年基金项目(21822804);国家自然科学基金杰出青年科学基金项目(21925802);NSFC-辽宁联合基金(U1908202,U1608222)。

摘　　要：铜酞菁是一类具有优异光物理性质和良好光热稳定性的染料,在印染、太阳能电池、传感器等领域中应用广泛。血清白蛋白作为血液中主要的转运蛋白,常用于小分子和药物的负载和运输。通过牛血清白蛋白(BSA)和铜酞菁类染料(LGS-CuPc)的自组装,构建了LGS-CuPc-BSA纳米粒子(LGS-CuPc-BSA NPs),研究了其作为光动力和光热一体化试剂在光疗中的作用。在671 nm光照下(800 mW·cm^−2),LGS-CuPc-BSA NPs活性氧产率达到23.3%,光热转换效率为36.8%。与LGS-CuPc比较,LGS-CuPc-BSA NPs光动力和光热治疗效果明显提升,且表现出较低的细胞毒性、良好的生物相容性以及在肿瘤细胞线粒体的定位能力,实现对肿瘤细胞的有效杀伤。Copper phthalocyanine is a kind of dye with excellent photophysical properties and good photothermal stability,and it has a wide range of applications in the fields of printing and dyeing,solar cells,sensors and so on.Serum albumin,as the main transport protein in the blood,is often used for loading and transporting small molecules and drugs.In this work,LGS-CuPC-BSA nanoparticles(NPs)were constructed by self-assemble of bovine serum albumin(BSA)and copper phthalocyanine dyes(LGS-CuPc),which works as an integrated reagent for photodynamic and photothermal therapy.Under illumination at 671 nm(800 mW·cm^−2),the reactive oxygen species yield of LGS-CuPc-BSA NPs reached 23.3%,and the photothermal conversion efficiency was 36.8%.Compared with LGS-CuPc,LGS-CuPc-BSA nanoparticles showed low cytotoxicity,good biocompatibility and mitochondrial localization in tumor cells.LGS-CuPc-BSA nanoparticles significantly promoted cell apoptosis in vitro,realizing the combination therapy of photothermal and photodynamic therapy.

关 键 词：铜酞菁 牛血清白蛋白 光热治疗 光动治疗 纳米材料 

分 类 号：TQ613.5[化学工程—精细化工]