类叶牡丹皂苷G在大鼠体内的组织分布研究  

Study on Distribution of Cauloside G from Caulophyllum robustum Maxim. in Tissues of Rats

作　　者：梁军[1] 宋艳 刘俊希 苑宏宇 黄晓莹[2] 夏永刚[1] 匡海学[1] LIANG Jun;SONG Yan;LIU Junxi;YUAN Hongyu;HUANG Xiaoying;XIA Yonggang;KUANG Haixue(Key Laboralory of Chinese Materia Medica,Ministry of Education,Heilongjiang Key Laboratory of Traditional Chinese Medicine and Natural Medicine Pharmacodynamic Material Bases,,Heilongjiang University of Chinese Medicine,Harbin 150040,China;The First Affiliatel Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学教育部北药基础与应用研究重点实验室,黑龙江省中药及天然药物药效物质基础研究重点实验室,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040

出　　处：《中医药学报》2021年第1期23-27,共5页Acta Chinese Medicine and Pharmacology

基　　金：黑龙江省自然科学基金面上项目(H2017063);黑龙江省应用技术研究与开发计划项目(GA19C104);黑龙江省普通本科高等学校青年创新人才(UNPYSCT-2016209);黑龙江省博士后基金项目(LBH-TZ20,LBH-Z15209);黑龙江中医药大学科研基金项目(201821)。

摘　　要：目的:建立了一种UPLC-MS/MS法,测定类叶牡丹皂苷G(Cauloside G)在大鼠体内各主要脏器(心、肝、脾、肾、脑)中的分布特点及组织药物浓度随时间变化的动态规律。方法:大鼠灌胃给予Cauloside G150 mg/kg,给药后0.5、1、2、4和6 h 5个时间点,收集心、肝、脾、肾、脑组织样品,处理后通过三重四级杆线性离子阱串联质谱仪分析。采用ACQUITY UPLC HSS T3(2.1 mm×150 mm,1.8μm)色谱柱,流动相0.1%甲酸水溶液-0.1%甲酸乙腈溶液,梯度洗脱,流速0.3 mL·min~(-1),电喷雾离子源,负离子模式,多重反应监测(MRM)。结果:大鼠灌胃给予Cauloside G后,Cauloside G在大鼠的心、肝、脾、肾和脑中均有分布。心脏组织中,Cauloside G是在给药0.5 h后浓度达到最高值,浓度为(53.7±7.60)ng/mL;肝、脾、肾、脑组织中,Cauloside G是给药1 h后浓度达到峰值,浓度分别为(90.1±6.19)ng/mL,(103.2±17.67)ng/mL,(101.8±14.32)ng/mL,(68.2±14.76)ng/mL。结论:Cauloside G在大鼠体内的各个组织均有分布,其中肝、脾、肾组织中药物浓度较高,该研究为类叶牡丹中三萜皂苷类成分的进一步开发和利用提供参考。Objective:To detect the distribution of Cauloside G in the main organs of the rats,including the heart,liver,spleen,kidney and brain,and to study the dynamic changes of drug concentration with time by UPLC-MS/MS.Methods:Rats were intragastrically administrated with 150 mg/kg Cauloside G.At the time of 0.5,1,2,4 and 6 h,the heart,liver,spleen,kidney and brain tissues of the rats were collected,and they were analyzed by UPLC-MS.The chromatography separation was performed on a ACQUITY UPLC HSS T3 column(2.1 mm×150 mm,1.8μm)with 0.1%formic acid aqueous solution and 0.1%formic acid acetonitrile solution for gradient elution(0.3 mL·min-1).Heated electrospray ionization source was applied in a negative ion mode for multiple reaction monitoring(MRM).Results:After intragastrical administration of Cauloside G,it was detected to be distributed in rat heart,liver,spleen,kidney and brain.The concentration of Cauloside G in rat heart peaked at 0.5 h(53.7±7.60)ng/mL.In addition,its concentration in liver(90.1±6.19)ng/mL,spleen(103.2±17.67)ng/mL,kidney(101.8±14.32)ng/mL and brain(68.2±14.76)ng/mL peaked at 1 h.Conclusion:Cauloside G is distributed in the main organs of the rats.Its concentration remains high in liver,spleen and kidney.The results provided references for the development and the application of triterpenoid saponins from Caulophyllum robustum Maxim.

关 键 词：类叶牡丹 Cauloside G 组织分布 超高效液相色谱-质谱 

分 类 号：R285.5[医药卫生—中药学]