鲜铁皮石斛对肺腺癌裸鼠移植瘤中自噬及抑癌基因表达的影响  

Effect of Fresh Dendrobium Officinalis on the Expression of Autophagy and Tumor Suppressor Gene in Lung Transplanted Tumor Tissues in Nude Mice

作　　者：庞晨 张锡流[1] 张秀玲 叶星江[1] 黄敏[2] PANG Chen;ZHANG Xiliu;ZHANG Xiuling;YE Xingjiang;HUANG Min(Department of Pathology,the First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning 530023,China;Department of Pharmacy,the First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning 530023,China)

机构地区：[1]广西中医药大学第一附属医院病理科,南宁530023 [2]广西中医药大学第一附属医院药学部,南宁530023

出　　处：《医药导报》2021年第2期163-169,共7页Herald of Medicine

基　　金：广西自然科学基金资助项目(2017GXNSFAA198175)。

摘　　要：目的探究鲜铁皮石斛对肺腺癌移植瘤的抑制情况及其机制。方法将30只裸鼠随机分为6组,空白对照组、模型对照组、环磷酰胺组、铁皮石斛大、中、小剂量组,每组5只。除空白对照组外,其他组建立肺腺癌裸鼠移植瘤模型。环磷酰胺组腹腔注射环磷酰胺0.025 g·kg^-1,每隔1 d给药,共13次;铁皮石斛小、中、大剂量组分别灌胃给予0.68 g·mL^-1铁皮石斛6.25,12.50,25.00 g·kg^-1,空白对照组和模型对照组灌胃0.9%氯化钠溶液,每天30 mL·kg^-1,连续25 d。用药4周后处死动物,免疫组化法检测肿瘤组织Beclin1和Ki67的阳性表达率,RT-PCR检测表皮生长因子(EGFR)、p16和p53的mRNA表达。结果与模型对照组比较,环磷酰胺组、铁皮石斛中、大剂量组Beclin1阳性表达率升高(P<0.05)。与模型对照组比较,环磷酰胺组和铁皮石斛大剂量组Ki67阳性表达率降低(P<0.05)。与模型对照组比较,环磷酰胺组和铁皮石斛大剂量组EGFR表达降低(P<0.05)。与模型对照组比较,环磷酰胺组、铁皮石斛中、大剂量组p16 mRNA表达升高(P<0.05)。与模型对照组比较,环磷酰胺组及铁皮石斛小、中、大剂量组p53 mRNA表达升高(P<0.05)。结论铁皮石斛可以抑制肺腺癌裸鼠移植瘤组织的生长,其作用机制可能与自噬相关因子Beclin1相关,通过Beclin1-p53和p16-Ki67相互协同作用以及调控与肿瘤血管生成相关因子EGFR表达量实现抑制移植瘤组织生长的目的。Objective To explore the inhibition of fresh Dendrobium officinalis on transplanted tumor tissues and its related mechanism.Methods A total of 30 of nude mice were randomly divided into 6 groups,including blank control group,model control group,cyclophosphamide group,Dendrobium officinalis low,medium and high-dose group.Except for blank control group,nude mice of the other group were establishment of A549 lung adenocarcinoma xenograft model.Nude mice in blank control group and model control group were given 0.9%sodium chloride solution(30 mL·kg^-1),those of cyclophosphamide group were given intraperitoneal injection of cyclophosphamide(every other day,0.025 g·kg^-1),those of Dendrobium officinalis low,medium and high-dose group were given Dendrobium officinalis 6.25,12.5,25 g·kg^-1.The time lasted 4 weeks,after the drug was stopped,the nude mice were sacrificed,and the tumor body was weighed and measured before use.Immunohistochemistry was used to observe the protein expression of autophagy related factor Beclin1 and proliferation index Ki67 in transplanted tumor tissues of nude mice.RT-PCR was used to detect the expression of tumor suppressor genes p53,p16 and EGFR in transplanted tumor tissues of nude mice.Results Compare with the model control group,the expression rate of Beclin1 in cyclophosphamide group,Dendrobium officinalis medium,high-dose group increased.Compared with the model control group,the positive expression rate of Ki67 in cyclophosphamide group and Dendrobium officinalis high-dose group decreased.Compared with the model control group,the expression of EGFR in the cyclophosphamide group and Dendrobium officinalis high-dose group were decreased(P<0.05).Compared with the model control group,the expression of p16 mRNA in the cyclophosphamide group,the Dendrobium officinalis medium,high-dose group were increased,and the difference were statistically significant(P<0.05).Compared with the model control group,p53 mRNA expression was increased in the cyclophosphamide group,Dendrobium officinalis low,m

关 键 词：铁皮石斛 肺癌 BECLIN1 表皮生长因子 

分 类 号：R286[医药卫生—中药学] R734.2[医药卫生—中医学]