GLP-1衍生物的电化学分析方法研究  

Study on the Electrochemical Analysis Method for GLP-1 Derivatives

作　　者：张军军 李馨恩 尤卓莹 桑延霞 Zhang Junjun;Li Xin’en;You Zhuoying;Sang Yanxia(College of Chemistry and Chemical Engineering,Guangdong Pharmaceutical University,Zhongshan 528458,China)

机构地区：[1]广东药科大学医药化工学院,广东中山528458

出　　处：《广东化工》2020年第24期18-19,35,共3页Guangdong Chemical Industry

基　　金：广东省自然科学基金项目(2015A030310015)。

摘　　要：利用溴甲酚紫与蛋白质的相互作用,本研究建立了一种溴甲酚紫为探针测定LDGLP-1多肽的方法,最佳条件下,溴甲酚紫峰电流的下降值与LDGLP-1浓度在0.8~10、10~40、40~90 mg/L范围内呈线性关系,其线性回归方程分别为ΔIp=0.2287C+0.0536、ΔIp=0.0958C+1.532、ΔIp=0.0181C+4.4957。该方法应用于LDGLP-1的定量分析准确可靠。Based on the interaction between bromocresol purple and proteins, the method for the determination of LDGLP-1 polypeptide was established in this study. Under these optimal conditions, the decline value of bromocresol violet peak current was linearly related to the concentration of LDGLP-1 in the range of 0.8~10, 10~40, 40~90 mg/L, and the linear regression equations were ΔIp=0.2287 C+0.0536, ΔIp=0.0958 C+1.532, ΔIp=0.0181 C+4.4957, respectively. This method is accurate and reliable for quantitative analysis of LDGLP-1.

关 键 词：GLP-1 电化学 溴甲酚紫 

分 类 号：TQ[化学工程]