黄连对2型糖尿病大鼠胰腺脂毒性及内质网应激相关蛋白表达的影响  

Effects of Rhizoma Coptidis on Lipotoxicity and Expression of Endoplasmic Reticulum Stress-Related Proteins in Pancreas from Rats with Type 2 Diabetes Mellitus

作　　者：詹玫琦 周珊珊[2] 万晓刚[3] ZHAN Mei-Qi;ZHOU Shan-Shan;WANG Xiao-Gang(The First Clinical Medical School,Guangzhou University of Chinese Medicine,Guangzhou 510450 Guangdong,China;Hubei University of Chinese Medicine,Wuhan 430065 Hubei,China;The First Affiliated Hospital,Guangzhou University of Chinese Medicine,Guangzhou 510405 Guangdong,China)

机构地区：[1]广州中医药大学第一临床医学院,广东广州510450 [2]湖北中医药大学,湖北武汉430065 [3]广州中医药大学第一附属医院,广东广州510405

出　　处：《广州中医药大学学报》2021年第1期123-129,共7页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省科技计划项目(编号:2015A020210092)。

摘　　要：【目的】观察中药黄连对高糖高脂饮食诱导的2型糖尿病大鼠胰腺脂毒性的调控作用及探讨相关内质网应激的作用机制。【方法】从100只Wistar大鼠中随机选取20只作为正常组给予普通饲料喂养,其余80只大鼠采用高脂高糖饲料喂养联合链脲佐菌素(STZ)注射方法建立2型糖尿病模型。将成模后的60只大鼠随机分为模型组、黄连组、二甲双胍组,每组各20只。黄连组、二甲双胍组大鼠分别对应灌胃给药5周,同期正常组和模型组给予相同体积的蒸馏水。5周后取血,分别测定大鼠空腹血糖(FBG)、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C)、游离脂肪酸(NEFA)水平,采用免疫组织化学法检测胰腺组织中葡萄糖调节蛋白78(GRP78)、C/EBP同源蛋白(CHOP)、活化转录因子4(ATF4)的表达。【结果】与正常组比较,模型组的FBG、TG、TC、LDL-C、NEFA水平明显升高(P<0.05),HDL-C水平明显下降(P<0.05),GRP78、CHOP、ATF4蛋白表达水平均明显升高;与模型组比较,黄连组和二甲双胍组FBG、TG、TC、LDL-C、NEFA水平明显下降(P<0.05),HDL-C水平明显升高(P<0.05),GRP78、CHOP、ATF4蛋白表达水平均显著降低。【结论】中药黄连能够降低2型糖尿病大鼠的血糖和血脂,有效改善大鼠糖脂代谢紊乱,减轻胰腺脂毒性程度,降低内质网应激GRP78、CHOP、ATF4蛋白的表达。Objective To observe the effects of Chinese herbal medicine Rhizoma Coptidis on regulating and controlling pancreatic lipotoxicity in rats with type 2 diabetes mellitus induced by high-sugar and high-fat diet and to explore the endoplasmic reticulum stress-related mechanism. Methods Twenty rats were randomly selected from100 Wistar rats as normal group,the other 80 rats were induced into type 2 diabetes mellitus model by feeding with high-fat, high-sugar diet and injection of streptozotocin(STZ). And then 60 successful modeling rats were randomly divided into model group,Rhizoma Coptidis group,Metformin group,20 rats in each group. Rhizoma Coptidis group and Metfeormin group were given intragastric administration of corresponding medicine for 5 weeks, and the normal group and model group were given intragastric administration of the same volume of distilled water during the same period. After 5 weeks of treatment, the levels of fasting blood glucose(FBG),triglyceride(TG), total cholesterol(TC), high density lipoprotein-cholesterol(HDL-C), low density lipoprotein-cholesterol(LDL-C), nonesterified fatty acid(NFFA)in serum were determined. The expression levels of glucose-regulated protein 78(GRP78),C/EBP homologous protein(CHOP)and activating transcription factor 4(ATF4)in the pancreas were determined by immunohistochemical method. Results Compared with the normal group,the levels of FBG,TG,TC,LDL-C and NEFA were increased in the model group,HDL-C level was decreased,expression levels of GRP78,CHOP and ATF4 were increased;Compared with the model group,the levels of FBG, TG, TC, LDL-C, NEFA were decreased in the Rhizoma Coptidis group and Metformin group,HDL-C level was increased,expression levels of GRP78,CHOP and ATF4 were decreased(P<0.05).Conclusion Chinese herbal medicine Rhizoma Coptidis is effective for lowering blood glucose and lipids in type 2 diabetes mellitus rats,improving glucose and lipid metabolism disorder,reducing pancreatic lipotoxicity,and decreasing the expression of endoplasmic reticulum stress

关 键 词：黄连 2型糖尿病 脂毒性 内质网应激 大鼠 

分 类 号：R285.5[医药卫生—中药学]