不同粒径微塑料颗粒亚急性染毒对雄性小鼠生殖毒性的影响  

The effect of sub-acute exposure of microplastics on the male mice reproductive function

作　　者：田坤明 陈秋 TIAN Kun-ming;CHEN Qiu(Department of environment health,School of public health,Zunyi Medical University,Zunyi Guizhou 563006,China)

机构地区：[1]遵义医科大学公共卫生学院环境卫生系,贵州遵义563006

出　　处：《毒理学杂志》2020年第6期492-495,496,共5页Journal of Toxicology

基　　金：遵义市校联合基金[(2019)43号];贵州省卫生健康科学基金(gzwjkj2019-1-215);学术青苗基金。

摘　　要：目的微塑料可在低等生物和水生生物中导致生殖毒性和胚胎发育障碍,本研究将进一步探讨其是否影响哺乳动物生殖功能。方法雄性C57BL/6小鼠分为4组,分别为对照(生理盐水)组、0.1、1和10μm组,每组10只小鼠。经灌胃染毒剂量为5 mg/kg·bw不同粒径微塑料颗粒28 d。染毒完毕后用透射电子显微镜观察睾丸超微结构损伤;苏木素-伊红(HE)染色分析睾丸组织病理改变;谷胱甘肽(GSH)、超氧化物歧化酶(SOD)和丙二醛(MDA)试剂盒检测睾丸组织氧化应激反应;精子HE染色观察精子质量。结果3个粒径微塑料均能造成睾丸生精细胞凋亡、自噬和内质网水肿,但是,精子质量未发生明显改变。此外,睾丸组织同样未发生明显病理改变,GSH、SOD和MDA检测同样发现睾丸组织未发生氧化应激反应。结论亚急性染毒微塑料可能会给雄性生殖功能造成潜在毒性,应进一步研究长期染毒纳米和微米尺度微塑料对雄性生殖功能的影响。Objective Previous studies indicated that MPs possess reproductive and developmental toxicity in lower organisms and aquatic organisms, to investigate whether MPs could generate reproductive toxicity in mammal animal. Methods Male C57 BL/6 mice were divided into four groups: control group(saline), 0.1 μm, 1 μm, and 10 μm group, and 10 mice per group, respectively. The mice were intragastric administration with 5 mg/kg·bw indicated microplastics for 28 days. The transmission electron microscopy was employed to observed the ultrastructural changes of testis;The pathological changes of testis were detected by HE staining. GSH, SOD, and MDA kidts were used to test the oxidative reaction of testis and HE staining of sperm to determine the quality of sperm. Results Three diameter-scale microplastics could result in the ultrastructural changes of spermatogonia, including apoptosis, autophagy, and endoplasmic reticulum edema after 28 days administration. However, it was not found that significant pathological changes in testis tissue. Additionally, the oxidative stress and sperm quality were also not influenced by the three diameter-scale microplastics. Conclusion Microplastics could lead to ultrastructural changes of testis, but not pathological alterations and reproductive toxicity.

关 键 词：微塑料 生殖毒性 氧化应激 

分 类 号：R114[医药卫生—卫生毒理学]