电针干预对早期MCAO大鼠步态的影响  

Effect of Electroacupuncture Intervention on Gait of Early-stage MCAO Rats

作　　者：李明哲 须燕玲[3] 张英杰 郑飞[1] 汪文静 徐鸣曙 单春雷[1] LI Ming-zhe;XU Yan-ling;ZHANG Ying-jie;ZHENG Fei;WANG Wen-jing;XU Ming-shu;SHAN Chun-lei(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,China;Renhe Hospital of Baoshan District,Shanghai 200431,China)

机构地区：[1]上海中医药大学,上海201203 [2]上海市针灸经络研究所,上海200030 [3]上海市宝山区仁和医院,上海200431

出　　处：《上海针灸杂志》2021年第1期95-100,共6页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金面上项目(81874035);上海市优秀学术带头人项目(19XD1403600);上海市卫生健康委智慧医疗专项(2018ZHYL0216);上海申康医院发展中心临床科技创新项目(SHDC12018126);国家重点研发计划(2018YFC2001600/04)。

摘　　要：目的建立大脑中动脉阻塞(MCAO)模型,动态观察电针干预对早期脑缺血大鼠步态的影响。方法24只雄性SD大鼠随机分为假手术组(S)、模型组(M)和电针组(EA),每组8只。制备MCAO大鼠模型后,EA组选取双侧曲池、足三里进行气麻下电针干预5 d。记录3组干预前后体质量、神经功能缺损评分(NDS)、脑血流、步态的变化。结果与S组比较,M组在D1、D5、D7时体质量显著下降(P<0.05);与M组比较,EA组D5、D7体质量显著上升(P<0.05)。与S组比较,M组各时间点NDS显著增加(P<0.05);与M组比较,EA组NDS在D5时显著减少(P<0.05)。与S组比较,M组脑血流量在D1、D5、D7时显著下降(P<0.05);与M组比较,EA组脑血流量在D1和D5时上升(P<0.05)。与S组比较,M组D1和D5时平均血流速度显著下降(P<0.05);与M组比较,EA组与D5时平均血流速度显著上升(P<0.05)。M组在D1时右前最大接触面积、压印面积均值、压印宽度均值、支撑相均值较其他两组显著增加(P<0.05);M组在D1时平均速度、右前步长均值较其他两组显著降低(P<0.05)。结论电针干预早期MCAO大鼠可促进步态的恢复。Objective By establishing a middle cerebral artery occlusion(MCAO)model,to observe the effect of acupuncture intervention on the gait of early-stage cerebral ischemia rats in a dynamic manner.Method Twenty-four male Sprague-Dawley(SD)rats were randomized into a sham-operation(S)group,a model(M)group and an electroacupuncture(EA)group,with 8 rats in each group.When the MCAO rat model was successfully established,the EA group was treated with RA intervention at bilateral Quchi(LI11)and Zusanli(ST36)under gas anesthesia for 5 d.The body weight,Neurological Deficit Score(NDS),cerebral blood flow and gait were observed before and after intervention in the three groups.Result Compared with the S group,the body weight decreased significantly at D1,D5 and D7 in the M group(P<0.05);compared with the M group,the body weight increased significantly at D5 and D7 in the EA group(P<0.05).Compared with the S group,the NDS increased significantly in the M group at every time point(P<0.05);compared with the M group,the NDS decreased significantly in the EA group at D5(P<0.05).Compared with the S group,the cerebral blood flow dropped significantly in the M group at D1,D5 and D7(P<0.05);compared with the M group,the cerebral blood flow increased in the EA group at D1 and D5(P<0.05).Compared with the S group,the mean blood flow velocity declined significantly in the M group at D1 and D5(P<0.05);compared with the M group,the mean blood flow velocity increased significantly in the EA group at D5(P<0.05).At D1,the right anterior maximum contact area,mean print area,mean print width and mean stance phase were significantly increased in the M group compared with the other two groups(P<0.05);the average speed and right anterior step length were significantly decreased in the M group at D1 compared with the other two groups(P<0.05).Conclusion EA intervention can boost the recovery of gait in early-stage MCAO rats.

关 键 词：电针 MCAO模型 步态分析 运动障碍 大鼠 

分 类 号：R2-03[医药卫生—中医学]