温针灸对膝骨关节炎大鼠P53、Map2k3和PGE_2的影响  

Effect of Warm Needling Moxibustion on P53,Map2k3 and PGE2 in Knee Osteoarthritis Rats

作　　者：王华敏[1] 宓轶群[1] 赵媛媛 张永亮[2] WANG Hua-min;MI Yi-qun;ZHAO Yuan-yuan;ZHANG Yong-liang(Shanghai Jiaotong University Sixth People’s Hospital,Shanghai 200233,China;Hebei Medical University Second Hospital,Shijiazhuang 050000,China)

机构地区：[1]上海交通大学附属第六人民医院,上海200233 [2]河北医科大学第二医院,石家庄050000

出　　处：《上海针灸杂志》2021年第1期101-106,共6页Shanghai Journal of Acupuncture and Moxibustion

基　　金：上海市卫生和计划生育委员会科研课题(201740034);上海市卫生和计划生育委员会中医药科技创新项目(ZYKC201701006);上海市徐汇区重要疾病联合攻关项目(XHLHGG201802)。

摘　　要：目的通过观察温针灸对KOA大鼠肿瘤抑制蛋白P53(P53)、促分裂原活化蛋白激酶3(Map2k3)mRNA和前列腺素E_2(PGE_2)蛋白含量的影响,探讨温针灸治疗KOA的可能机制。方法将28只健康的雄性SD大鼠随机分为正常组、模型组、药物组和温针灸组,每组7只。正常组常规饲养,余3组大鼠采用关节腔注射木瓜蛋白酶papain联合强迫运动造模处理。造模成功后,温针灸组大鼠选用双侧"膝前穴";药物组大鼠予美洛昔康药物治疗。治疗结束后通过HE染色观察大鼠滑膜组织形态学变化,ELISA检测大鼠血清PGE_2蛋白含量,实时荧光定量PCR法检测滑膜组织中P53和Map2k3 mRNA的表达情况。结果HE染色结果显示与正常组相比,模型组可见明显的炎性细胞浸润,药物组可见少量炎性细胞、部分脂肪细胞和增生的血管,温针灸组可见少量炎性细胞散在分布。与正常组比较,模型组、药物组和温针灸组大鼠血清PGE_2含量、滑膜组织P53 mRNA表达显著升高(P<0.01);与模型组比较,温针灸组、药物组血清PGE_2蛋白含量、滑膜组织P53 mRNA表达水平明显降低(P<0.05);温针灸组与药物组比较差异无统计学意义(P>0.05)。各组大鼠滑膜组织中Map2k3 mRNA表达比较差异无统计学意义(P>0.05)。结论温针灸在一定程度上可以减少滑膜组织炎性细胞数量,通过降低PGE_2的含量抑制炎性反应。温针灸能够降低KOA大鼠滑膜组织P53 mRNA的异常高表达。Objective To explore the possible mechanism of warm needling moxibustion treatment for knee osteoarthritis through observing its effect on tumor suppressor protein P53(P53),mitogen-activated protein kinase 3(Map2k3)mRNA and prostaglandin E2(PGE2)contents in knee osteoarthritis rats.Method Twenty-eight healthy male SD rats were randomized to normal,model,medication and warm needling moxibustion groups,seven each.The normal group was fed routinely.In the other three groups of rats,the model was made by Intraarticular injection of papain plus forced movement.After successful model making,the warm needling moxibustion group of rats was treated with bilateral"anterior knee point"and the medication group of rats,with meloxicam.After the completion of treatment,rat morphological changes in synovial membrane were observed by HE staining,serum PGE2 content was measured by ELISA and the expressions of P53 and Map2k3 mRNAs in synovium were determined by real-time fluorescent quantitative PCR assay.Result HE staining results showed that inflammatory cell infiltration was obviously visible in the model group,a few inflammatory cells,partial adipocytes and hyperplastic blood vessels were visible in the medication group and a few inflammatory cells were scatteredly distributed in the warm needling moxibustion group,compared with the normal group.Serum PGE2 content and the expression of P53 mRNA in synovium increased significantly in the model,medication and warm needling moxibustion groups of rats compared with the normal group(P<0.01)and decreased significantly in the warm needling moxibustion and medication groups compared with the model group(P<0.05);there were no statistically significant differences between the warm needling moxibustion and medication groups(P>0.05).There was no statistically significant difference in the expression of Map2k3 mRNA in synovium between various groups of rats(P>0.05).Conclusion Warm needling moxibustion can decrease the number of inflammatory cells in synovium to a certain extent and inhibit

关 键 词：灸法 温针灸 骨关节炎 膝 肿瘤抑制蛋白P53 促分裂原活化蛋白激酶3 前列腺素E_2 

分 类 号：R2-03[医药卫生—中医学]