NOD1和NOD2在Hp阳性胃癌发生、发展中的作用机制研究  

Mechanism of NOD1 and NOD2 in pathogenesis and development of Hp positive gastric cancer

作　　者：申学东 王红卫 呼延智伟 SHEN Xuedong;WANG Hongwei;HU Yanzhiwei(Department of Outpatient,the First Mobile Corps of Armed Police Hospital,Dingzhou,Hebei 073000,China;Department of Gastroenterology,the First People′s Hospital of Jinzhong,Shanxi 030600,China)

机构地区：[1]武警第一机动总队医院门诊部,河北定州073000 [2]山西省晋中市第一人民医院消化科,030600

出　　处：《重庆医学》2021年第1期13-16,21,共5页Chongqing medicine

摘　　要：目的研究核苷酸结合寡聚化结构域(NOD)1和NOD2蛋白在幽门螺杆菌(Hp)阳性胃癌发生、发展中的机制。方法分别采用免疫组织化学和实时荧光定量聚合酶链反应检测NOD1和NOD2蛋白、mRNA的表达水平,并将Hp阴性和阳性胃癌组的结果进行比较;采用Western blot检测Hp阳性的早期和中晚期胃癌组天冬氨酸蛋白水解酶-3(caspase-3)和核因子-κB(NF-κB)的表达水平。结果Hp阴性和Hp阳性胃癌组间NOD1和NOD2的蛋白表达水平差异均有统计学意义(P<0.05),其中Hp阳性胃癌组中NOD1和NOD2蛋白均呈高表达;Hp阴性和Hp阳性胃癌组间NOD1和NOD2 mRNA的表达水平差异均有统计学意义(P<0.05),其中Hp阳性胃癌组中NOD1和NOD2 mRNA呈高表达;Hp阳性的早期和中晚期胃癌组间caspase-3和NF-κB的表达水平差异均有统计学意义(P<0.05),中晚期胃癌组caspase-3和NF-κB表达水平均高于早期胃癌组。结论Hp阳性胃癌组织NOD1和NOD2的表达增加,二者可能通过激活NF-κB通路和凋亡因子caspase-3,在胃癌的发展中发挥着促凋亡作用。Objective To study the mechanism of nucleotide oligomerization domain(NOD)1 and NOD2 in the pathogenesis and development of Hp positive gastric cancer.Methods The protein and mRNA expression levels of NOD1 and NOD2 were detected by immunohistochemistry and real-time fluorescence quantitative PCR,respectively,and the results of Hp negative and positive gastric cancer groups were compared.And the expression levels of caspase-3 and NF-κB in early and middle-advanced Hp positive gastric cancer group were detected by Western blot.Results There were significant differences in the expressions of NOD1 and NOD2 protein between Hp negative and Hp positive gastric cancer group(P<0.05),and the expressions of NOD1 and NOD2 protein were high in Hp positive gastric cancer group.There were significant differences in expressions of NOD1 and NOD2 mRNA between the two groups(P<0.05),in which NOD1 and NOD2 mRNA expressions were high in Hp positive gastric cancer group.There were significant differences in expressions of caspase-3 and NF-κB between in early and middle-advanced Hp positive gastric cancer group(P<0.05),in which the expression of caspase-3 and NF-κB in the middle-advanced Hp positive gastric cancer group was higher than that in the early gastric cancer group.Conclusion The expressions of NOD1 and NOD2 increased in Hp positive gastric cancer.Both of them may play a role in promoting apoptosis in the development of gastric cancer by activating NF-κB pathway and apoptosis factor caspase-3.

关 键 词：核苷酸结合寡聚化结构域1 核苷酸结合寡聚化结构域2 幽门螺杆菌 胃肿瘤 机制 

分 类 号：R735.2[医药卫生—肿瘤]