AtEXD参与植物盐胁迫的作用研究  

Research on AtEXD Participating in Response to Salt Stress in Plant

作　　者：张香香 滕炎桐 陈涛 ZHANG Xiangxiang;TENG Yantong;CHEN Tao(Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院生物技术研究所,北京100081

出　　处：《生物技术进展》2021年第1期61-68,共8页Current Biotechnology

基　　金：中央级公益性科研院所基本科研业务费项目(Y2019XK15-04);新疆维吾尔自治区重点研发任务专项(201809274)。

摘　　要：在植物的生长发育过程中,各种逆境胁迫作为环境因素对植物产生了很多不良反应,其中盐胁迫一直都是对植物的生长发育和作物产量影响最大的非生物胁迫之一。在对拟南芥盐胁迫的研究中,发现了1个盐胁迫应答基因AtEXD(At2g25910),通过对其进行基本特性分析了解到AtEXD是1个具有3’-5’外切酶结构域和KH结构域的蛋白,在进化关系上具有较高的保守性,但目前对其功能知之甚少。酵母双杂交实验筛选到了一些与AtEXD相互作用的转录因子,功能分析结果显示AtEXD很可能参与植物体内的逆境胁迫应答机制。种子萌发率统计实验结果显示,突变体exd-1和exd-2具有盐敏感表型,而AtEXD过表达时,拟南芥的耐盐性明显增强。研究结果表明AtEXD很可能正调控植物的耐盐性。Various stresses have bad effects on plants in the process of plant growth and development.Among them,salt stress has always been one of the abiotic stresses that have the worst impact on plant growth and crop yield.When studying Arabidopsis salt stress,we discovered gene AtEXD(At2g25910)responding to salt stress.By the analysis of its basic characteristics,we learned that AtEXD had a 3′-5′exonuclease domain and a KH domain and were highly conservative in evolutionary relationships.In contrast,we know very little about its function now.By the yeast two-hybrid,we screened some transcription factors interacting with AtEXD and the results of functional analysis showed that AtEXD might be involved in the stress response mechanism of plants.The statistical results of seeds germination rate showed that the mutants exd-1 and exd-2 had salt-sensitive phenotypes.When AtEXD was overexpressed,the salt tolerance of Arabidopsis was significantly enhanced.These results indicated that AtEXD probably regulate the salt tolerance of plants.

关 键 词：拟南芥 AtEXD 酵母双杂交 盐胁迫 

分 类 号：Q945[生物学—植物学] S567.7[农业科学—中草药栽培]