脑心肌炎病毒VP1蛋白抑制Ⅰ型IFN信号通路和促进病毒体外增殖  

VP1 protein of encephalomyocarditis virus inhibits type Ⅰ IFN signaling pathway and promotes virus proliferation in vitro

作　　者：韩玉梅 谢晶莹[3] 毕英杰 许淑娟 冯若飞[1] HAN Yumei;XIE Jingying;BI Yingjie;XU Shujuan;FENG Ruofei(Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Biomedical Research Center, Northwest Minzu University, Lanzhou 730030, China;College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, China;College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China)

机构地区：[1]西北民族大学生物医学研究中心生物工程与技术国家民委重点实验室,甘肃兰州730030 [2]西北民族大学生命科学与工程学院,甘肃兰州730030 [3]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《浙江农业学报》2021年第1期18-26,共9页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(31460665);2019年中央专项研究生科研创新项目(Yxm2019148);国家民委中青年英才计划[(2018)98];教育部“创新团队发展计划”(IRT_17R88)。

摘　　要：脑心肌炎病毒是一种重要的人畜共患病病原,为了初步研究EMCV介导的天然免疫反应的分子机制,将VP1蛋白克隆至真核表达载体pCMV-HA,转染HEK293细胞后,使其在细胞内过表达,通过ELISA和实时荧光定量PCR检测VP1过表达对IFN-β表达和RLRs信号通路相关因子,以及下游刺激基因表达的影响。结果表明,VP1蛋白可显著抑制IFN-β的表达。进一步研究发现,VP1可显著抑制EMCV引起的RLRs信号通路相关因子mRNA水平(P<0.01),并且随着VP1的表达,MAVS蛋白的表达明显减少,但对MDA5表达无影响。实验数据初步表明,VP1蛋白可以通过抑制MAVS的表达,进而抑制Ⅰ型IFN基因的表达并阻断其下游信号通路发挥作用。Encephalomyocarditis virus(EMCV)is an important zoonotic pathogen.In order to preliminarily study the molecular mechanism of EMCV mediated innate immune response,this study cloned VP1 protein into eukaryotic expression vector pCMV-HA,transfected it into HEK293 cells to make it overexpress in cells.The effects of VP1 overexpression on IFN-βexpression and RLRs signaling pathway-related factors and downstream stimulation gene expression were detected by ELISA and real time fluorescence quantitative PCR.Results showed VP1 could significantly inhibit IFN-βexpression.Further study found that VP1 could significantly inhibit the mRNA level of EMCV-induced RLRs signaling pathway-related factors,and with the expression of VP1,MAVS protein expression was significantly reduced,but had no effect on the expression of MDA5.Therefore,the data of this experiment preliminarily showed that VP1 protein could inhibit the expression of typeⅠIFN gene expression and block its downstream signaling pathway by inhibiting the expression of MAVS.

关 键 词：脑心肌炎病毒 结构蛋白VP1 Ⅰ型IFN信号通路 

分 类 号：S855.99[农业科学—临床兽医学]