利用超临界CO2萃取法提取西藏白花杜鹃花中活性成分及GC-MS初步分析  

Supercritical Carbon Dioxide Extraction for Flowers of Rhododendron mucronatum(Blume)G.Don and Preliminary Analysis of Chemical Composition by Gas Chromatography Mass Spectrometry(GC-MS)

作　　者：吴金措姆[1] 四郎玉珍[1] 班旦[1] 格桑卓嘎[1] 郝宝成[2] 拉巴次旦[1] Wujincuomu;Silangyuzhen;Bandan;Gesangzhuoga;HAO Bao-cheng;Labacidan(Institute of Animal Science and Veterinary,Tibet Academy of Agricultural and Animal Husbandry Sciences,Tibet Lhasa 850000,China;Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agriculture Sciences,Gansu Lanzhou 730050,China)

机构地区：[1]西藏自治区农牧科学院畜牧兽医研究所,西藏拉萨850000 [2]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050

出　　处：《西藏农业科技》2020年第4期34-37,共4页Tibet Journal of Agricultural Sciences

基　　金：西藏自治区财政涉农推广类项目:藏兽药研发与示范。

摘　　要：确定超临界CO2萃取西藏白花杜鹃花的最优工艺条件和初步鉴定其提取物的化学成分。以提取率为评价指标,利用正交试验对西藏白花杜鹃花超临界CO2萃取有效成分条件进行了优化,采用气相色谱-质谱联用技术(gas chromatgraphy-mass spectrometry,GC-MS)分析了萃取物的化学成分。结果表明,超临界CO2萃取西藏白花杜鹃花的最优提取工艺为开花期白花杜鹃的花粉碎为200目、萃取压力25 MPa、萃取温度为40℃、反应时间2.5 h,GC-MS检测分离出24种含量较高的化合物。由此可得,经与质谱谱图数据库比对,初步鉴定主要为烷烃及其含氧衍生物(62.32%)、烯和醇(8.09%)、酯(4.87%)等化合物,其中含有提高机体免疫力和抗肿瘤作用的角鲨烯(含量达4.79%)等药用活性成分,具有很好的研究开发前景。The present paper aimed to determine the optimal extraction process of the flowers of Rhododendron mucronatum(Blume)G.Don,and preliminarily identify and analyze the main chemical components of the extract.Taking the extraction rate as the evaluation index,the flowers of Rhododendron mucronatum(Blume)G.Don were extracted with supercritical carbon dioxide by orthogonal optimization method.The chemical components of the extract were analyzed by gas chromatography mass spectrometry(GC-MS).Results showed that the optimum conditions for supercritical carbon dioxide extraction of the flowers of Rhododendron mucronatum(Blume)G.Don were as follows:crushing into 200 mesh,extraction pressure 25 MPa,extraction temperature 40℃,reaction time 2.5 hours.24 compounds with high content were detected and separated by GC-MS.To conclude,compared with the mass spectrum database,supercritical carbon dioxidextraction for the flowers of Rhododendron mucronatum(Blume)G.Don was mainly composed of Alkanes and their oxygen-containing derivatives(62.32%),alkenes and alcohols(8.09%),esters(4.87%),etc.

关 键 词：白花杜鹃 超临界CO2萃取法 化学成分 GC-MS 

分 类 号：R284.[医药卫生—中药学]