Gelatin-based hydrogels combined with electrical stimulation to modulate neonatal rat cardiomyocyte beating and promote maturation  

作　　者：Feng Zhang Kaiyun Qu Xiaopei Li Chaoming Liu Lazarus S.Ortiz Kaihong Wu Xiaowei Wang Ningping Huang 

机构地区：[1]State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Si Pai Lou 2#,Nanjing 210096,China [2]Cardiovascular Center,Children’s Hospital of Nanjing Medical University,Nanjing 210008,China [3]Department of Cardiovascular Surgery,The First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China

出　　处：《Bio-Design and Manufacturing》2021年第1期100-110,共11页生物设计与制造（英文）

基　　金：This work was financially supported by the National Natural Science Foundation of China(Grant No.31871017);the Natural Science Foundation of Jiangsu Province,China(Grant No.BK20171352);the Southeast University-Nanjing Medical University Cooperative research project(2242019K3DN05);the Medical Science and Technology Development Foundation,Jiangsu Provincial Commission of Health and Family Planning,China(ZDRCA2016073);the“111”Project(B17011,Ministry of Education of China).

摘　　要：Cardiovascular diseases are the leading cause of morbidity and mortality throughout the world underlining the importance of efficient treatments including disease modeling and drug discovery by cardiac tissue engineering.However,the predictive power of these applications is currently limited by the immature state of the cardiomyocytes.Here,we developed gelatin hydrogels chemically crosslinked by genipin,a biocompatible crosslinker,as cell culture scaffolds.Neonatal rat cardiomyocytes appear synchronous beatingwithin 2 days after seeding on hydrogels.Furthermore,we applied the electrical stimulation as a conditioning treatment to promote the maturation of cardiomyocytes cultured on the hydrogels.Our results show that electrical stimulation improves the organization of sarcomeres,establishment of gap junctions,calcium-handling capacity and propagation of pacing signals,thereby,increase the beating velocity of cardiomyocytes and responsiveness to external pacing.The above system can be applied in promoting physiological function maturation of engineered cardiac tissues,exhibiting promising applications in cardiac tissue engineering and drug screening.

关 键 词：Gelatin hydrogel Electrical stimulation Cardiomyocyte maturation Cardiac tissue engineering 

分 类 号：TQ427.26[化学工程] R318.08[医药卫生—生物医学工程]