Ni^2+对钝顶螺旋藻生长及藻胆蛋白含量的影响  

Effects of Nickel(Ni^2+)on Growth,Spectral Characteristics and Phycobiliprotein Content of Alga Spirulinaplatensis

作　　者：赵嶝科 刘慧[2] 张少斌[1] 陈小杏 刘金洲 辛桥 ZHAO Dengke;LIU Hui;ZHANG Shaobin;CHEN Xiaoxing;LIU Jinzhou;XIN Qiao(College of Biosciences and Biotechnology,Shenyang Agricultural University,Shenyang 110866,China;College of Land and Environment,Shenyang Agricultural University,Shenyang 110866,China)

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110866 [2]沈阳农业大学土地与环境学院,辽宁沈阳110866

出　　处：《水产科学》2021年第1期128-132,共5页Fisheries Science

基　　金：国家级大学生创新创业项目(201910157006);沈阳农业大学大学生创新创业项目(2019396).

摘　　要：为研究Ni^2+对螺旋藻生长的影响,采用室内培养法,在培养液中添加不同质量浓度的Ni^2+,对螺旋藻的生长曲线、生物量、吸收光谱以及藻胆蛋白含量进行了测定和分析。试验结果显示,当Ni^2+质量浓度为0~0.5μg/mL时,随着质量浓度的增加,藻胆蛋白含量增加,螺旋藻生长加快,累积的螺旋藻生物量增加;0.5μg/mL的Ni^2+对螺旋藻生长、生物量累积和藻胆蛋白含量具有最大促进作用;0.5μg/mL的Ni^2+处理,导致螺旋藻叶绿素a在蓝紫光区的吸收光谱发生明显变化,440 nm的吸收峰消失,而在430 nm和450 nm处分别出现一个吸收峰;Ni^2+对藻蓝蛋白和别藻蓝蛋白的吸收光谱没有显著影响。当Ni^2+质量浓度达到1.0μg/mL时,螺旋藻仍能正常生长。试验结果表明,0.5μg/mL的微量Ni^2+通过改变螺旋藻叶绿素a在蓝紫光区的吸收光谱,提高藻胆蛋白含量,从而促进螺旋藻的生长和生物量的累积,是促进螺旋藻生长的最佳Ni^2+质量浓度,为降低螺旋藻生产成本,以及利用螺旋藻治理水体镍污染提供科学依据。In order to study the effect of Ni^2+on growth of alga Spirulina platensis,the growth curve,biomass,absorption spectrum and phycobiliprotein content of the alga were determined and analyzed by adding different concentrations of Ni^2+to the culture medium.The results showed that at Ni^2+concentration of 0—0.5μg/mL,with the increase in Ni^2+concentration,the content of phycobiliprotein increased,the growth of accelerated and the accumulated biomass of Spirulina was increased,the greatest promoting effect at 0.5μg/mL Ni^2+.When treated with 0.5μg/mL Ni^2+,the absorption spectrum of chlorophyll a in blue and purple region of the alga were changed obviously,the absorption peak at 440 nm disappeared and there were two absorption peak around 430 nm and 450 nm,respectively.Ni^2+had no significant effect on the absorption spectrum of phycocyanin and allophycocyanin.When the Ni^2+concentration of 1.0μg/mL,the alga still grew normally.It is concluded that 0.5μg/mL trace Ni^2+is the best concentration to promote the growth and biomass accumulation of spirulina by increasing the content of phycobilin and changing the absorption spectrum of chlorophyll a in the blue and purple region.The finding provides the basis for reducing the production cost of Spirulina and controlling the nickel pollution of the water body by using the spirulina.

关 键 词：钝顶螺旋藻 镍 生长 吸收光谱 藻胆蛋白 

分 类 号：S917.3[农业科学—水产科学]