低剂量电离辐射对STZ诱导糖尿病模型大鼠海马神经元的影响  

Effect of low dose ionizing radiation on hippocampal neurons in STZ-induced diabetic rats

作　　者：刘飞 梁硕[1] 王栎萱[1] 秦丽晶 郭伟 王志成[1] LIU Fei;LIANG Shuo;WANG Yuexuan;QIN Lijing;GUO Wei;WANG Zhicheng(Key Laboratory of Radiobiology of NHC,School of Public Health,Jilin University,Changchun 130021,China;Second Department of Outpatients,Armed Police Jilin General Hospital,Changchun 130052,China)

机构地区：[1]吉林大学公共卫生学院国家卫健委放射生物学重点实验室,吉林长春130021 [2]武警吉林总队医院第二门诊部,吉林长春130052

出　　处：《吉林大学学报（医学版）》2021年第1期1-7,共7页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金项目(81803218);吉林省科技厅科技发展计划项目(20190201202JC,20180101305JC);吉林大学创新训练项目(202010183303)。

摘　　要：目的:探讨低剂量电离辐射(LDR)对糖尿病模型大鼠海马神经元细胞周期、钙离子浓度([Ca^2+]i)和[线粒体膜电位(MMP)]的影响,阐明LDR对糖尿病神经损伤的保护作用机制。方法:利用链脲佐菌素(STZ)建立Wistar大鼠糖尿病(DM)模型。60只大鼠分为正常对照组、DM组、DM+25 mGy X射线照射组、DM+50 mGy X射线照射组和DM+75 mGy X射线照射组,每组12只。除正常对照组和DM组外,其余各组大鼠分别给予25、50和75 mGy X射线隔日照射,共计12次,并监测大鼠体质量。继续饲养4周后,麻醉处死大鼠,取出各组大鼠海马后制备单细胞悬液,利用流式细胞术检测大鼠不同细胞周期海马神经元百分率、[Ca^2+]i和MMP。结果:正常对照组大鼠体质量随着时间的延长逐渐增加;与正常对照组比较,DM组、DM+25 mGy X射线照射组、DM+50 mGy X射线照射组和DM+75 mGy X射线照射组大鼠体质量明显降低(P<0.05);与DM组比较,DM+50 mGy X射线照射组和DM+75 mGy X射线照射组大鼠体质量明显增加(P<0.05)。与正常对照组比较,DM组大鼠中S期海马神经元百分率和[Ca^2+]i明显增加(P<0.05),海马神经元MMP明显降低(P<0.05);与DM组比较,DM+50 mGy X射线照射组和DM+75 mGy X射线照射组大鼠S期海马神经元百分率和[Ca^2+]i明显降低(P<0.05),海马神经元MMP明显升高(P<0.05);与DM+25 mGy X射线照射组比较,DM+75 mGy X射线照射组大鼠海马神经元中[Ca^2+]i明显降低(P<0.05),DM+50 Gy X射线照射组和DM+75 mGy X射线照射组海马神经元MMP明显升高(P<0.05)。结论:LDR可通过改善DM所致大鼠海马神经元细胞周期S期延迟、[Ca^2+]i升高和MMP降低,进而发挥神经保护作用。Objective:To investigate the effects of low dose ionizing radiation(LDR)on the cell cycle,calcium ion concentration([Ca^2+]i)and mitochondrial membrane potential(MMP)of hippocampal neurons in the diabetic rats,and to elucidate the protective mechanisms of LDR on nerve injury.Methods:The Wistar rat models of diabetes mellitus(DM)were established with streptococci(STZ).Sixty rats were divided into normal control group,DM X-ray irradiation group,DM+25 mGy X-ray irradiation group,DM+50 mGy X-ray irradiation group and DM+75 mGy X-ray irradiation group;there were 12 rats in each group.Except normal control and DM groups,the rats in other groups were administered with 25,50 and 75 mGy LDR every other day for 12 times,and the body weights of the rats were monitored.After 4 weeks of feeding,the rats were sacrificed after anesthesia.The hippocumpus tissue of the rats in various groups was removed and single cell suspension was prepared.The percentages of hippocampal neurons at different cell cycles,([Ca^2+]i)and MMP were detected by flow cytometry,respectively.Results:With the prolongation of time,the body weight of the rats in normal control group was increased gradually.Compared with normal control group,the body weights of diabetic rats in DM group,DM+25 mGy X-ray irradiation group,DM+50 mGy X-ray irradiation group and DM+75 mGy X-ray irradiation group were significantly decreased(P<0.05);compared with DM group,the body weights in DM+50 mGy group and DM+75 group mGy group were significantly increased(P<0.05).Compared with normal control group,the percentages of hippocampal neurons in S phase and[Ca^2+]i of the rats in DM group were increased significantly(P<0.05),while MMP was decreased significantly(P<0.05);compared with DM group,the percentages of hippocampal neurons in S phase and[Ca^2+]i of the rats in DM+50 mGy X-ray irradiation group and DM+75 mGy X-ray irradiation group were decreased significantly(P<0.05),while MMP of hippocampal neurons was increased significantly(P<0.05);compared with DM+25 mGy X-ray irradiati

关 键 词：低剂量辐射 糖尿病 海马神经元 细胞周期 线粒体膜电位 

分 类 号：Q691[生物学—生物物理学] R587.1[医药卫生—内分泌]