辛伐他汀联合机械牵拉激活Wnt/β⁃catenin信号通路促进大鼠骨髓间充质干细胞成骨分化  

Simvastatin promotes the osteogenic differentiation of BMSCs induced by mechanical stretch via Wnt/β-catenin signaling pathway

作　　者：陈斌伟[1] 刘圣曜 黄帅 王斌[1] 陈元鸿 CHEN Binwei;LIU Shengyao;HUANG Shuai;WANG Bin;CHEN Yuanhong(The Second Affiliated Hospital qf Guangzhou Medical University,Guangzhou 510260,China)

机构地区：[1]广州医科大学附属第二医院,广州510260

出　　处：《实用医学杂志》2021年第1期41-45,共5页The Journal of Practical Medicine

基　　金：广东省自然科学基金资助项目(编号:2020A1515010211);广州市卫生健康科技项目(编号:20191A011081)。

摘　　要：目的探讨辛伐他汀联合机械牵拉对骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)成骨分化的影响及Wnt/β-catenin通路在其中的作用。方法实验分为对照组、辛伐他汀组、机械牵拉组及辛伐他汀+机械牵拉组,利用碱性磷酸酶(ALP)活性及RT-PCR检测各组BMSCs的ALP活性及ALP、OCN、Col 1a、RUNX2基因表达,利用RT-PCR及Western blot检测β-catenin通路相关基因和蛋白的表达,并探讨DKK1处理后辛伐他汀联合机械牵拉对成骨分化的影响。结果与对照组比,辛伐他汀、机械牵拉及辛伐他汀+机械牵拉均能增加ALP活性及成骨基因表达,并能增加β-catenin基因和蛋白表达,且以辛伐他汀+机械牵拉组升高最明显;使用Wnt/β-catenin信号通路抑制剂DKK1能减弱辛伐他汀+机械牵拉组促成骨分化效果。结论辛伐他汀及机械牵拉均促进BMSCs成骨分化,且辛伐他汀协同机械牵拉通过激活Wnt/β-catenin通路促进BMSCs成骨分化。Objective To investigate the effect of simvastatin combined with mechanical stretch on osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)and explore its mechanisms.Methods simvastatin group,mechanical stretch group and simvastatin+mechanical stretch group in the study.ALP activity and RT-PCR were used to investigate the effect of simvastatin,mechanical stretch and simvastatin+mechanical stretch on osteogenic differentiation of BMSCs and RT-PCR and WB were used to investigate the expression of β-catenin in each group.Results The level of osteogenic relative genes was up-regulated and the expression ofβ-catenin protein was increased in each group when compared with those in the control group,especially in simvastatin+mechanical stretch group.DKK1 partly eliminated osteogenic differentiation of BMSCs in simvastatin+mechanical stretch group.Conclusion Both simvastatin and mechanical stretch promote the osteogenic differentiation of BMSCs.Simvastatin promotes the osteogenic differentiation of BMSCs induced by mechanical stretch via Wnt/β-catenin signaling pathway.

关 键 词：骨髓间充质干细胞 辛伐他汀 机械牵拉 成骨分化 

分 类 号：R319[医药卫生—基础医学]