枸杞水提液对海洛因视网膜氧化损伤的影响  

The Effect of Lycium barbarum Soup on Oxidative Damage of Heroin to Retina

作　　者：贾宗平 苟陶然[2] 俞诗源 JIA Zongping;GOU Taoran;YU Shiyuan(Department of Public Security and Technology,Gansu University of Political Science and Law,Lanzhou 730070,China;College of Life Science,Northwest Normal University,Lanzhou 730070,China)

机构地区：[1]甘肃政法大学公安技术学院,兰州730070 [2]西北师范大学生命科学学院,兰州730070

出　　处：《中国细胞生物学学报》2020年第12期2141-2149,共9页Chinese Journal of Cell Biology

基　　金：兰州市社会发展项目(批准号:2014-1-102);甘肃省高等学校科研项目(批准号:2014A-086)资助的课题。

摘　　要：该研究探讨了枸杞水提液对海洛因视网膜氧化损伤的影响。实验将120只昆明小鼠随机分为对照组、海洛因组、枸杞1组和枸杞2组。用递增剂量的海洛因溶液对海洛因组、枸杞1组、枸杞2组小鼠腹腔连续注射15天(1~5天、6~10天、11~15天分别注射2.0 g/L、3.0 g/L、4.0 g/L的海洛因溶液,每次0.2 mL,每天两次),对照组注射等量生理盐水。注射海洛因溶液1 h后,枸杞1组、枸杞2组再分别灌胃0.2 mL枸杞水提液(枸杞1组、枸杞2组分别对应0.2 g/mL、0.4 g/mL的枸杞水提液),对照组和海洛因组再分别灌胃等量生理盐水。称量小鼠体质量和眼珠重的变化,用比色法检测小鼠血浆GSH含量、LDH活性和视网膜组织SOD活性、MDA含量的变化,用免疫组织化学法检测视网膜组织Bax、TNF-α蛋白表达的变化。结果表明,与对照组相比,海洛因组小鼠体质量和眼球重降低,血浆GSH含量显著下降,LDH活性显著升高,视网膜组织SOD活性降低,MDA含量升高,视网膜组织Bax和TNF-α蛋白的阳性表达量升高,平均光密度值明显增大;与海洛因组相比,枸杞组小鼠体质量和眼球重有所增加,血浆GSH含量有所升高,LDH活性显著降低,视网膜组织SOD活性增加,MDA含量降低,视网膜组织Bax和TNF-α蛋白阳性表达有所降低。综上,枸杞水提液能提高视网膜细胞的抗氧化能力和抗炎能力,抑制视网膜组织Bax和TNF-α蛋白的阳性表达,对视网膜组织有一定的保护作用。The aim of this study was to explore the effects of Lycium barbarum soup on oxidative damage of heroin to retina.A total of 120 Kunming mice were randomly divided into control group,heroin group,L.barbarum 1 group and L.barbarum 2 group.The mice in heroin group,L.barbarum 1 group,and L.barbarum 2 group were intraperitoneally injected with increasing dose of heroin solution for 15 days (1-5,6-10,and 11-15 days correspond to 2.0 g/L,3.0 g/L,and 4.0 g/L heroin solution respectively,0.2 mL each time,twice a day).In the control group,the same amount of normal saline was injected.After injecting heroin solution for 1 h,L.barbarum 1,2 groups were given with 0.2 mL L.barbarum soup (L.barbarum 1 group and L.barbarum 2 group correspond to 0.2 g/mL and 0.4 g/mL L.barbarum soup respectively) by intragastric administration.The control group and heroin group were given with the same amount of normal saline.The changes of body mass and eyeball weight of the mice were measured.The changes of GSH content and LDH activity in plasma,SOD activity and MDA content in retina were detected by colorimetry,and the levels of Bax and TNF-α proteins in retina were detected by immunohistochemistry.The results showed that compared with the control group,the body mass and eyeball weight of the mice in heroin group decreased,the content of GSH in plasma decreased significantly,the LDH activity of plasma increased significantly,the activity of SOD in retina decreased,the content of MDA increased,the positive level of Bax and TNF-α protein in retina increased,and the average optical density increased significantly.Compared with the heroin group,the body mass and eyeball weight of the mice in L.barbarum groups increased again,the content of GSH in plasma increased,the LDH activity of plasma decreased significantly,the activity of SOD in retina increased,the content of MDA decreased,and the levels of Bax and TNF-α proteins in retina decreased again.In conclusion,L.barbarum soup can improve the anti-oxidation and anti-inflammatory abilities of ret

关 键 词：枸杞水提液 海洛因 视网膜 SOD BAX TNF-α LDH 免疫组织化学 

分 类 号：R285[医药卫生—中药学]