青霉菌灭活菌丝体提取物对烟草BY-2细胞抗病防卫反应的诱导作用  

Extracts of dry mycelium of Penicillium chrysogenum-induced defense responses in tobacco BY-2 cells

作　　者：钟宇 黄坤[3] 李妤[2] 付坚[4] 陈壮壮[5] 刘春明 王建光[2] 陈穗云[2] ZHONG Yu;HUANG Kun;LI Yu;FU Jian;CHEN Zhuangzhuang;LIU Chunming;WANG Jianguang;CHEN Suiyun(College of Agronomy and Life Sciences,Kunming University,Kunming 650214,China;School of Ecology and Environmental Science,Crop Disease&Pest Biocontrol Engineering Research Center of Yunnan Province,Yunnan Plant Disease&Pest Biocontrol Engineering Research Center of Institutions of Higher Education,Yunnan University,Kunming 650091,China;Yunnan Tobacco Company Honghe Branch,Mile,Yunnan 652399,China;Institute of Biotechnology and Germplasm Resources,Yunnan Academy of Agricultural Sciences,Kunming 650205,China;College of Bio-Ecological Engineering,Dongying Vocational Institute,Dongying,Shandong 257091,China)

机构地区：[1]昆明学院农学与生命科学学院,昆明650214 [2]云南大学生态与环境学院,云南省作物病虫害生物防控工程技术研究中心,云南省高校植物病虫害生物防控工程技术中心,昆明650091 [3]云南省烟草公司红河州公司,云南弥勒652399 [4]云南省农业科学院生物技术与种植资源研究所,昆明650205 [5]东营职业学院生物与生态工程学院,山东东营257091

出　　处：《植物生理学报》2020年第11期2477-2486,共10页Plant Physiology Journal

基　　金：云南省基础研究计划项目(2018FA022);国家自然科学基金(31660038);云南省烟草公司科技计划项目(2018-530000241013)。

摘　　要：青霉菌灭活菌丝体水提取物(water extract of dry mycelium of Penicillium chrysogenum, DMP)可以作为激发子诱导植物抗病防卫反应。本研究对DMP中多糖(PsD)和多肽(PDMP)激发子进行分离提取,并以chitin和Flg22为参照,研究Ps D和PDMP对烟草BY-2细胞抗病防卫反应的诱导作用。结果表明:PsD和PDMP能够诱导BY-2细胞活性氧迸发,分别在处理后5和25 min达到峰值。PsD和PDMP能够诱导烟草BY-2细胞苯丙烷途径关键基因PAL、4CL和C4H和几丁质基因NtCHitIV的表达,并积累酚类次生代谢产物。基础免疫标志基因Pti5表达量在Ps D和PDMP诱导下显著升高,说明Ps D和PDMP与植物细胞互作的机理可能为模式识别受体介导的MAMP识别,并诱导下游抗病防卫反应。以上结果表明, PsD和PDMP能够激活烟草BY-2细胞基础抗性,诱导抗性防卫反应。Water extract of dry mycelium of Penicillium chrysogenum(DMP) serves as an elicitor to induce plant defense responses. In this study, the polysaccharide(PsD) and polypeptide(PDMP) were isolated from DMP, and the effects of PsD and PDMP on defense responses in tobacco BY-2 cells were studied, using chitin and Flg22 as references. The results showed, PsD and PDMP induced oxidative burst in tobacco BY-2 cells, the peaks were observed 5 min after PsD treatment, and 25 min after PDMP treatment, respectively. Transcripts of key regulators genes of phenylpropanoid pathway, PAL, 4CL, and C4H, as well as a chitinase gene NtCHitIV, were rapidly induced both in PsD and PDMP treated cells. Transcripts of marker gene of MAMP/PAMP-triggered immunity(PTI) Pti5 was rapidly induced both in PsD and PDMP treated cells, indicating that PsD and PDMP actor as MAMP to trigger PTI, and activate following defense responses. This study suggests that PsD and PDMP act as elicitors and activate defense responses though PTI in tobacco BY-2 cell.

关 键 词：青霉菌 烟草BY-2细胞 微生物相关分子模式 基础免疫 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]