淫羊藿苷对家兔退变腰椎间盘组织中Ⅱ型胶原和蛋白多糖表达的影响  

Effect of Icariin on the Expression of Type Ⅱ Collagen and Proteoglycan in Rabbits’ Degenerative Lumbar Disc Tissues

作　　者：贺宪[1] 曾展鹏[1] 孔畅[1] 直彦亮[1] 马志锋[1] 翁茂鉴 HE Xian;ZENG Zhanpeng;KONG Chang;ZHI Yanliang;MA Zhifeng;WENG Maojian

机构地区：[1]广州市番禺区中医院骨二区,广东广州511400

出　　处：《新中医》2020年第24期1-4,共4页New Chinese Medicine

基　　金：广东省中医药局科研项目(20181217)。

摘　　要：目的:观察淫羊藿苷对家兔退变腰椎间盘组织中Ⅱ型胶原和蛋白多糖表达的影响,探讨淫羊藿苷防治腰椎间盘退变的作用机制。方法:用随机数字表法将30只家兔分为实验组、模型组和空白组,每组10只。实验组和模型组采用髓核穿刺抽吸法复制腰椎间盘退变模型。术后4周,实验组按3 mg/(kg·d)的剂量灌胃淫羊藿苷,模型组和空白组用等量生理盐水灌胃,连续4周。术后4周和术后8周,各组分别取5只用空气栓塞法处死后切取椎间盘组织。HE染色观察其组织病理学变化,免疫组织化学法检测Ⅱ型胶原的表达水平,间苯三酚分光光度法检测蛋白多糖的含量。结果:术后4周,空白组髓核组织内基质成分高,密度均匀,染色深,其间有大量髓核细胞,核深染,胞浆淡染;实验组和模型组髓核内基质及髓核细胞部分消失,被深染的纤维组织和梭形的成纤维细胞取代。术后8周,空白组髓核成分同术后4周比无明显变化;而模型组髓核的基质及髓核细胞大部分消失,染色变浅,大量成纤维细胞和纤维组织形成;实验组髓核基质成分较高,髓核细胞减少。术后4周,与空白组比较,模型组Ⅱ型胶原、蛋白多糖表达水平降低(P<0.05)。术后8周,与空白组比较,模型组Ⅱ型胶原、蛋白多糖表达水平明显降低(P<0.05);与模型组比较,实验组Ⅱ型胶原、蛋白多糖表达水平升高(P<0.05)。结论:淫羊藿苷可通过上调Ⅱ型胶原的表达、增加蛋白多糖的含量来延缓腰椎间盘退变。Objective:To observe the effect of icariin on the expression of typeⅡ collagen and proteoglycan in rabbits’ degenerative lumbar disc tissues, and to explore the mechanism of icariin in the prevention and treatment of lumbar disc degeneration. Methods:A total of 30 rabbits were divided into the experiment group,the model group and the blank group according to the random number table method,10 rabbits in each group. The experiment group and the model group was adopted a method of puncture and aspiration of the nucleus pulposus to replicate models with lumbar disc degeneration. Four weeks after the operation,the experiment group received icariin at a dose of 3 mg/(kg·d),and the model group and the blank group received the same amount of normal saline by gavage;all groups were intervened for four weeks. Four weeks and eight weeks after the operation,five rabbits in every group were selected and sacrificed by air embolization to obtain their lumbar discs. The histopathological changes were observed from HE staining;the expression levels of type Ⅱ collagen was determined by immunohistochemistry,and the content of proteoglycan was determined by phloroglucinol spectrophotometry.Results:Four weeks after the operation,there was a high content of matrix in the nucleus pulposus of the blank group with uniform density and deep staining, contained with a lot of nucleus pulposus cells whose nucleus was hyperchromatic and cytoplasm lightly stained. In the experiment group and the model group, the matrix in the nucleus pulposus and nucleus pulposus cells partially disappeared and were replaced by hyperchromatic fibrous tissue and fusiform fibroblasts. Eight weeks after the operation,there was no significant changes in the content of nucleus pulposus when compared to four weeks after the operation in the blank group;in the model group,most of the matrix in the nucleus pulposus and nucleus pulposus cells disappeared,the staining became lighter,and a large number of fibroblasts and fibrous tissue formed;in the experiment grou

关 键 词：腰椎间盘退变 淫羊藿苷 Ⅱ型胶原 蛋白多糖 动物实验 家兔 

分 类 号：R285.5[医药卫生—中药学]