HPLC法同时测定丝棉木中2种成分含量  

Determination of Two Chemical Constituents in Euonymus Maackii by HPLC

作　　者：庞策 赵鹏慧 张冠腾 左亚奇 丁孟 郭晶晶 甄攀[2] PANG Ce;ZHAO Peng-hui;ZHANG Guan-teng;ZUO Ya-qi;DING Meng;GUO Jing-jing;ZHEN Pan(Department of Pharmacy,Hebei North University,Zhangjiakou,075000,China;Institute of Applied Chemistry,Hebei North University,Zhangjiakou,075000,China)

机构地区：[1]河北北方学院药学系,张家口075000 [2]河北北方学院应用化学研究所,张家口075000

出　　处：《神经药理学报》2020年第2期15-18,共4页Acta Neuropharmacologica

基　　金：河北省高等学校科学技术研究项目(No.ZD2016016);2018年度河北北方学院项目(No.YB2018030)。

摘　　要：目的:建立同时测定丝棉木中卫矛羰碱(compound 1)和6α,12-二乙酸基-1β,9α-二乙酸(β-呋喃甲酸酯基)-4α-羰基-2β-2-甲基丁酯-β-二氢沉香呋喃(compound 2)含量的高效液相色谱(HPLC)法,测定丝棉木叶、种子和茎中这2种成分的含量。方法:石油醚提取,挥干石油醚,甲醇溶解,HPLC法检测。Syncronis C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(72∶28),流速1.0 mL·min-1,紫外检测波长为254 nm,柱温25℃。结果:compound 1和compound 2的线性范围分别为0.17~2.72 mg·mL-1(r1=0.9997)和0.10~1.60 mg·mL-1(r2=0.9995);检出限分别为10.3μg·mL-1、13.8μg·mL-1;平均加样回收率分别为96.1%~110.7%、99.6%~103.5%。丝棉木叶中compound 1含量范围0.02~0.03 mg·g-1,compound 2未检出;种子中compound 1和compound 2含量范围分别为1.64~3.06 mg·g-1、0.53~1.41 mg·g-1;茎中compound 1和compound 2均未检出。结论:建立的高效液相色谱法简便、快捷、稳定、准确,可用于测定丝棉木中2种成分含量。同一株丝棉木的叶、种子、茎中2种化合物含量不同;不同株丝棉木的相同部位2种成分含量差异显著。Objective:To establish methods to determine contents of evonine(compound 1)and 6α,12-diacetoxy-1β,9αdi(β-furancarbonyl-oxy)-4α-hydroxy-2β-2-methylbutanoyloxy-β-dihydroagarofuran(compound 2)simultaneously in Euonymus maackii by high performance liquid chromatography(HPLC),to determine the contents of two components in leaves,seeds and stems of Euonymus maackii.Methods:Extraction by petroleum ether,drying of petroleum ether,methanol dissolution,detection by HPLC.Syncronis C18 chromatographic column(250 mm×4.6 mm,5μm)was adopted.The mobile phase was methanol-water(72∶28),the flow rate was 1.0 mL·min-1,the detection wavelength was 254 nm,and the column temperature was 25℃.Results:The linear range of compound 1 and compound 2 was respectively 0.17~2.72 mg·mL-1(r1=0.9997)、0.10~1.60 mg·mL-1(r2=0.9995),the detection limit was respectively 10.3μg·mL-1 and 13.8 μg·mL-1,the average recovery rate was 96.1% to 110.7%,99.6% to 103.5%,respectively.The content range of compound 1 in leaves of Euonymus maackii was 0.02~0.03 mg·g-1,andcompound 2 was not detected. The contents of compound 1 and 2 in seeds ranged from 1.64 to3.06 mg·g-1 and from 0.53 to 1.41 mg·g-1,respectively. Compounds 1 and 2 in stem were notdetected. Conclusion:HPLC method is simple,fast,stable and accurate,which can be used todetect two chemical components in Euonymus maackii. Contents of two chemical compoundsin leaves,seeds and stems of the same plant were different,and contents of two chemicalcomponents in the same parts of different plants were significantly different.

关 键 词：丝绵木 卫矛羰碱 二氢沉香呋喃 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]