盐碱培养条件对几种外生菌根真菌生长的影响  

Effect of Saline-alkali Conditions on Several Ectomycorrhizal Fungi in vitro

作　　者：张健[1,2] 谷口武士 徐明 邹晓 山中典和[2] ZHANG Jian;TANIGUCHI Takeshi;XU Ming;ZOU Xiao;YAMANAKA Norikazu(Institute of Fungal Resources,Institute of Edible Fungus,School of Life Science,Guizhou University,Guiyang 550025,Guizhou,China;Arid Land Research Center,Tottori University,Tottori 6800001,Japan)

机构地区：[1]贵州大学生命科学学院,真菌资源研究所,食用菌研究院,贵州贵阳550025 [2]鸟取大学干燥地研究中心,日本鸟取6800001

出　　处：《食用菌学报》2021年第1期61-65,共5页Acta Edulis Fungi

基　　金：国家自然科学基金(31660150、31860037);贵州省科技计划{黔科合基础[2017]1059;黔科合支撑[2018]2773;黔科合重大专项字[2019]3005-4;黔科合平台人才[2017]5788号};贵州省一流学科建设项目{GNYL[2017]009}。

摘　　要：为探讨盐碱培养条件对外生菌根真菌生长的影响,选取了土生空团菌(Cenococcum geophilum)、威氏盘菌(Wilcoxina mikolae)、硬皮地星(Astraeus hygrometricus)、彩色豆马勃(Pisolithus tinctorius)、须腹菌(Rhizopogon luteolus)、黏盖乳牛肝菌(Suillus bovinus)、点柄乳牛肝菌(S.granulatus)和棉革菌属(Tomentella sp.)菌株为研究对象,设置不同碱性条件(pH为8、9和10)和不同盐胁迫条件(NaCl和CaCl_2分别为0.1 mol·L^-1和0.2 mol·L^-1)的液体酵母浸膏葡萄糖培养基,pH 7为对照组,5个重复,经过42 d暗培养测定培养基的pH和菌丝体生物量。不同外生菌根真菌对盐碱培养条件的耐受程度不同,适应盐碱培养条件最强的菌株为威氏盘菌,其次是棉革菌属;在pH 7时所有菌株均可生长;当pH 8时,仅威氏盘菌能够生长,菌丝体生物量为(2.883±0.396)mg·mL^-1;威氏盘菌在0.1 mol·L^-1和0.2 mol·L^-1 CaCl2培养条件下也能够生长,菌丝体生物量分别为(5.300±0.371)mg·mL^-1和(4.584±0.481)mg·mL^-1;威氏盘菌在0.1 mol·L^-1 NaCl培养条件下生长受到一定影响,菌丝体生物量为(1.581±0.520)mg·mL^-1;在0.1 mol·L^-1 CaCl_2培养条件下棉革菌属生长受到明显影响,菌丝体生物量为(1.175±0.838)mg·mL^-1;在0.1 mol·L^-1 NaCl培养条件下棉革菌属能微弱生长,其菌丝体生物量为(0.077±0.053)mg·mL^-1。The effect of saline-alkali conditions on growth of ectomycorrhizal fungi was studied using Cenococcum geophilum,Wilcoxina mikolae,Astraeus hygrometricus,Amanita ibotengutake,Pisolithus tinctorius(3 strains:Pt1,Pt2 and Pt3),Rhizopogon luteolus,Suillus bovinus,S.granulatus(2 strains:Sg1 and Sg2),and Tomentella sp.as test subjects.Different saline-alkali conditions were set up as follows:alkaline conditions at pH 8,pH 9 and pH 10,respectively;salt conditions at 0.1 and 0.2 mol·L-1 NaCl and 0.1 and 0.2 mol·L-1 CaCl2.The neutral pH 7 condition was the control and each treatment condition had 5 replicates.After cultivation in darkness for 42 d,the biomass of ectomycorrhizal fungi and the medium pH in each treatment group were measured.The results showed that W.mikolae had the strongest adaptability to saline-alkali conditions,followed by Tomentella sp..All the tested ectomycorrhizal fungi grew under pH 7.At pH 8,only W.mikolae showed growth with(2.883±0.396)mg·mL-1 biomass,which was significantly reduced compared with the control.There was no growth for any tested ectomycorrhizal fungus at pH 9.There was no growth in the tested ectomycorrhizal fungi under CaCl2 except W.mikolae[(5.300±0.371)and(4.584±0.481)mg·mL-1 at 0.1 and 0.2 mol·L-1 CaCl2,respectively]and Tomentella sp.[(1.175±0.838)mg·mL-1 at 0.1 mol·L-1CaCl2].Interestingly,addition of 0.1 mol·L-1 CaCl2 promoted the biomass of W.mikolae and 0.2 mol·L-1CaCl2 had no effect on the biomass of W.mikolae.With the addition of 0.1 mol·L-1 NaCl,only W.mikolae and Tomentella sp.showed limited growth with reduced biomass of(1.581±0.520)and(0.077±0.053)mg·mL-1,respectively.None of the tested ectomycorrhizal fungi had mycelial growth at 0.2 mol·L-1 NaCl.

关 键 词：菌根真菌 PH 盐碱 

分 类 号：Q935[生物学—微生物学]