蒙古口蘑和干巴菌水提物对四氯化碳诱导小鼠急性肝损伤的保护作用  

Water Extracts of Tricholoma mongolicum and Thelephora ganbajun Protected Mice from CCl4-induced Acute Hepatotoxicity

作　　者：张梦萍 贾凤兰[1] 张宝旭[1] ZHANG Mengping;JIA Fenglan;ZHANG Baoxu(Department of Toxicology,School of Public Health,Peking University/Key Laboratory of Chinese Medicine Compatibility and Attenuation of the State Administration of Traditional Chinese Medicine/Beijing Key Laboratory of Food Safety Toxicology Research and Evaluation,Beijing 100191,China)

机构地区：[1]北京大学公共卫生学院毒理系,国家中医药管理局中药配伍减毒重点研究室,食品安全毒理学研究与评价北京市重点实验室,北京100191

出　　处：《食用菌学报》2021年第1期66-74,共9页Acta Edulis Fungi

基　　金：国家重点研发计划(2018YFD0400201)。

摘　　要：通过皮下注射四氯化碳橄榄油溶液建立小鼠急性肝损伤模型,分别灌胃蒙古口蘑(Tricholoma mongolicum)水提物(剂量分别为7、14、28 g·kg^-1)和干巴菌(Thelephora ganbajun)水提物(剂量分别为8、16、24 g·kg^-1),通过测定肝脏系数、血清中谷丙转氨酶(ALT)和乳酸脱氢酶(LDH)的含量,制备HE染色切片观察肝组织病理变化,研究蒙古口蘑和干巴菌水提物对小鼠急性肝损伤的保护作用。结果表明:与模型组比较,蒙古口蘑水提物低剂量组ALT含量显著降低,中、高剂量组极显著降低;高剂量组LDH含量极显著降低。干巴菌水提物中、高剂量组ALT含量显著降低;中、高剂量组LDH含量极显著降低。蒙古口蘑和干巴菌水提物各剂量组ALT和LDH含量均随着给药剂量的增加而降低,具有剂量效应关系。蒙古口蘑和干巴菌水提物各剂量组的肝损伤程度随着给药剂量的增加而逐渐减轻。研究结果表明,蒙古口蘑和干巴菌水提物对四氯化碳诱导的小鼠急性肝损伤具有一定的保护作用。Male SPF grade ICR mice were randomly divided into eight groups with five mice in each group as follows:control group,model group,Tricholoma mongolicum water extract groups(low,medium and high doses),and Thelephora ganbajun water extract groups(low,medium and high doses).The acute liver injury model was established by subcutaneous injection of carbon tetrachloride(CCl4)olive oil solution.Then mice with acute liver injury were gavaged with water extract of T.mongolicum at doses of 7,14 and 28 g·kg-1and water extract of T.ganbajun at doses of 8,16 and 24 g·kg-1,respectively.Meanwhile,mice in the control and the model groups were gavaged with distilled water.After treatment for 6 d,mice in each group were measured for relative liver weight,serum alanine aminotransferase(ALT)and lactate dehydrogenase(LDH),and HE stained tissue sections were prepared to observe pathological changes in liver tissue.Compared with the model group,ALT contents in T.mongolicum water extract groups and the LDH content of the high dose T.mongolicum water extract group were significantly decreased;ALT and LDH contents in both medium and high dose T.ganbajun water extract groups were significantly decreased.Both ALT and LDH contents in water extract groups decreased with increased dose,suggesting a dose-effect relationship.HE staining of liver tissue showed that both T.mongolicum and T.ganbajun water extracts ameliorated liver damage on a dose dependent manner.The results suggested that water extracts of T.mongolicum and T.ganbajun protected mice from acute liver injury induced by carbon tetrachloride.

关 键 词：蒙古口蘑 干巴菌 急性肝损伤 谷丙转氨酶 乳酸脱氢酶 

分 类 号：R285.5[医药卫生—中药学]